Cytokine Mix E is a ready-to-use mixture of growth factors optionally available for the HPC Expansion Medium DXF (C-28021). The animal component-free, defined formula contains recombinant human TPO, SCF, flt-3 ligand and IL-3. The optimized formulation allows vigorous expansion of hematopoietic progenitor cells, while maintaining their primitive phenotype. Differentiation is largely inhibited.
Quality
All lots of PromoCell Hematopoietic Progenitor Media are subjected to comprehensive quality control tests using primary human mononuclear cells as well as purified hematopoietic progenitor cells from cord blood. Each lot of PromoCell HPC Expansion Medium DXF is tested for the ability to support the expansion of hematopoietic progenitor cells with a primitive phenotype. Each lot of PromoCell Hematopoietic Progenitor Medium is tested for the capacity to induce directed differentiation into hematopoietic lineages using purified CD34+ progenitor cells from cord blood. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).
Storage and Stability
Store the Cytokine Mix at -20°C in the dark immediately after arrival. If stored properly, the it is stable until the expiry date stated on the label. Medium supplemented with Cytokine Mix E should be used within 2 weeks after addition of the cytokines. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.
CD34+ Progenitor Cells are suitable for diverse studies, as well as for the directed differentiation into more committed blood cell phenotypes and endothelial lineages.
Hematopoietic stem cell culture protocols for isolation, expansion, and differentiation of CD34+ cells including CFU assays.
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