C-39165
Airway Epithelial Cell Growth Medium
SupplementMix containing all media supplements pre-mixed in one vial, 1 Mix for 500 ml
Synonym(s):
Recommended with C-21260
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.81
Recommended Products
form
aqueous solution
packaging
pkg of 1 vial
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−20°C
General description
Airway Epithelial Cell Growth Medium SupplementMix (basal medium sold separately)
To form a complete medium, it should be combined with the basal medium: C-21260
To form a complete medium, it should be combined with the basal medium: C-21260
Application
Airway Epithelial Cell Growth Medium is a serum-free medium optimized for the in vitro cultivation of epithelial cells from large air passages. The medium is optimized for primary human cells, but can also be used for equine, porcine, murine, bat and rat airway epithelial cells.Recommended for:Human Nasal Epithelial Cells (HNEpC)Human Bronchial Epithelial Cells (HBEpC)Human Tracheal Epithelial Cells (HTEpC)
Components
Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials
Quality
All lots of PromoCell Airway Epithelial Cell Growth Medium are subjected to comprehensive quality control tests using primary human airway epithelial cells. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested airway epithelial cells. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).
Preparation Note
Final supplement concentrations after addition to the basal medium:Bovine Pituitary Extract: 0.004 ml / ml Epidermal Growth Factor (recombinant human): 10 ng / mlInsulin (recombinant human): 5 µg / mlHydrocortisone: 0.5 µg / mlEpinephrine: 0.5 µg / mlTriiodo-L-thyronine: 6.7 ng / mlTransferrin, holo (human): 10 µg / mlRetinoic Acid: 0.1 ng / ml
Storage and Stability
Store the SupplementMix at -20°C in the dark immediately after arrival. If stored properly, it is stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.
Subculture Routine
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recommended
Product No.
Description
Pricing
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
Optimize primary human airway epithelial cell culture with serum-free media and handling protocols for large air passage cells.
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