The majority of our basal media have a shelf life of 9 months from the date of manufacture when stored between 4°C – 8°C. The shelf life of the supplements is 12 months from the date of manufacture when stored at -20°C. The exact expiry date is specified on the product label.
C-23025
Fibroblast Growth Medium 3
Ready-to-use kit including Basal Medium and SupplementMix, 500 ml
Synonym(s):
Primary Cell Culture Media
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500 ML
$228.00
About This Item
UNSPSC Code:
12352207
NACRES:
NA.71
Recommended Products
form
liquid
packaging
pkg of 500 ml
technique(s)
cell culture | mammalian: suitable
shipped in
wet ice
General description
Fibroblast Growth Medium 3 (Ready-to-use)
Application
PromoCell Fibroblast Growth Medium 3 has been developed for the in vitro cultivation of adult Human Cardiac Fibroblasts (HCF). The medium is optimized for primary human cells but can also be used for porcine cardiac fibroblasts.Recommended for:Human Cardiac Fibroblasts (HCF)
Components
Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials
Quality
All lots of PromoCell Fibroblast Growth Medium 3 are subjected to comprehensive quality control tests using primary Human Cardiac Fibroblasts. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested fibroblasts. Approved inhouse lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).
Preparation Note
Final supplement concentrations after addition to the basal medium:Fetal Calf Serum: 0.1 ml / mlBasic Fibroblast Growth Factor (recombinant human): 1 ng / ml Insulin: 5 µg / ml
Storage and Stability
Store the Basal Medium at 4 to 8°C in the dark and the SupplementMix or SupplementPack at -20°C immediately after arrival. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.Note: The SupplementMix and SupplementPack are delivered thawed and can be frozen after arrival without losing any activity.
Subculture Routine
Click here for more information.
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Articles
Primary human fibroblasts and growth medium for the in vitro cultivation of juvenile and adult fibroblasts. Protocols for handling, cell passaging, media, and product use.
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Could you please provide the shelf life of Product No. C-23025, PromoCell FIBROBLAST GROWTH MEDIUM 3?
1 answer-
Helpful?
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I will be using cardiac fibroblasts and aside from normal growth media (full amount of Serum) I have to serum starve the cells before my experiments. What media can I purchase where I can only change the amount of serum, not other supplements?
1 answer-
This product does contain serum in the formulation. A pre-formulated supplement is included with the medium that contains serum, bFGF, and insulin. The supplement, as well as any additional serum, may be added or withheld at the end user's discretion. Product 116F-500, an alternative fibroblast growth medium that does not contain serum that may be suitable for serum-starved experimental conditions, may also be of interest. Please see the link below to review this product option:
https://www.sigmaaldrich.com/product/sigma/116f500Helpful?
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