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AV32557

Sigma-Aldrich

Anti-GFI1 (AB2) antibody produced in rabbit

affinity isolated antibody

Synonym(s):

Anti-Growth factor independent 1 transcription repressor, Anti-ZNF163

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

conjugate

unconjugated

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

45 kDa

species reactivity

pig, bovine, rat, human

concentration

0.5 mg - 1 mg/mL

technique(s)

western blot: suitable

NCBI accession no.

NP_001120687

UniProt accession no.

Q99684

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... GFI1(2672)

General description

Gfi-1 is a zinc finger repressor that inhibits the proliferation and conserves functional integrity of hematopoietic stem cells. Human GFI1 mutations have been linked to neutropenia.
Rabbit Anti-GFI1 (AB1) antibody recognizes human, mouse, rat, and canine GFI1.

Immunogen

Synthetic peptide directed towards the N terminal region of human GFI1

Application

Rabbit Anti-GFI1 (AB2) antibody can be used for western blot applications at a concentration of 1μg/ml.

Biochem/physiol Actions

GFI1 may be a transcription factor involved in regulating the expression of genes active in the S phase during cell cycle progression in T-cells. GFI1 may be involved in tumor progression. Defects in GFI1 are a cause of autosomal dominant severe congenital neutropenia (SCN) and dominant nonimmune chronic idiopathic neutropenia of adults (NI-CINA) This gene encodes a nuclear zinc finger protein that functions as a transcriptional repressor. This protein plays a role in diverse developmental contexts, including hematopoiesis and oncogenesis. It functions as part of a complex along with other cofactors to control histone modifications that lead to silencing of the target gene promoters. Mutations in this gene cause autosomal dominant severe congenital neutropenia, and also dominant nonimmune chronic idiopathic neutropenia of adults, which are heterogeneous hematopoietic disorders that cause predispositions to leukemias and infections. Multiple alternatively spliced variants, encoding the same protein, have been identified for this gene.

Sequence

Synthetic peptide located within the following region: VPAPSRADSTSNAGGAKAEPRDRLSPESQLTEAPDRASASPDSCEGSVCE

Physical form

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Hanno Hock et al.
Nature, 431(7011), 1002-1007 (2004-10-01)
Haematopoietic stem cells (HSCs) sustain blood production throughout life. HSCs are capable of extensive proliferative expansion, as a single HSC may reconstitute lethally irradiated hosts. In steady-state, HSCs remain largely quiescent and self-renew at a constant low rate, forestalling their
Richard E Person et al.
Nature genetics, 34(3), 308-312 (2003-06-05)
Mice lacking the transcriptional repressor oncoprotein Gfi1 are unexpectedly neutropenic. We therefore screened GFI1 as a candidate for association with neutropenia in affected individuals without mutations in ELA2 (encoding neutrophil elastase), the most common cause of severe congenital neutropenia (SCN;

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