A9357

Anti-Actin, Cardiac antibody, Mouse monoclonal

clone AC1-20.4.2, purified from hybridoma cell culture

• Synonym(s): Anti-ACTC, Anti-ACTC1, Anti-Actin alpha cardiac muscle 1, Anti-CMD1R, Anti-CMH11
• UNSPSC Code: 12352203
• NACRES: NA.41
• conjugate: unconjugated
• application: ELISA (i); ICC; IHC (p); WB
• clone: AC1-20.4.2, monoclonal
• species reactivity: rat, human, bovine, chicken, mouse
• citations: 27
• technique(s): immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 10-20 μg/mL using digested rat heart tissue; indirect ELISA: suitable; western blot: suitable

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: AC1-20.4.2, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~42 kDa
• species reactivity: rat, human, bovine, chicken, mouse
• concentration: ~1.0 mg/mL
• technique(s): immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 10-20 μg/mL using digested rat heart tissue; indirect ELISA: suitable; western blot: suitable
• isotype: IgG1
• UniProt accession no.: P68032
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... ACTC1(70) ; mouse ... Actc1(11464) ; rat ... Actc1(29275)

Description

General description

Actins are cytoskeletal proteins that are widely expressed in eukaryotes. These proteins primarily regulate various cellular functions such as muscle contraction, mRNA synthesis and apoptosis among many others. ACTC1 is the predominant actin isoform form in adult cardiac tissues and alterations in this isoform have been associated with hypertrophic cardiomyopathy and congenital heart disease . Monoclonal Anti-Actin, Cardiac antibody is specific for human, bovine, chicken, rat, and mouse cardiac α actin.

Immunogen

synthetic NH₂-terminal decapeptide of cardiac isoform of actin.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Western Blotting (1 paper)

Monoclonal Anti-Actin, Cardiac antibody is suitable for use in immunocytochemistry, immunoblot (approx. 42 kDa), indirect ELISA, and western blot. The antibody may also be used in immunohistochemistry (using formalin-fixed, paraffin-embedded sections, or using a conc. of 10-20 μg/mL in digested rat heart tissue).

Protein samples isolated from cardiac sections or cardiac myocytes were subjected to western blot using monoclonal mouse anti-actin antibody as the primary at a dilution of 1:2000.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)