A8850
Auto-Activated Protein Kinase from bovine kidney
from bovine kidney, aqueous solution
Synonym(s):
AK
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
Recommended Products
biological source
bovine kidney
Quality Level
description
Minimum 50 ng protein/vial by Bradford
form
aqueous solution
specific activity
~17,000 units/mg protein
mol wt
36 kDa
shipped in
dry ice
storage temp.
−70°C
Biochem/physiol Actions
This enzyme phosphorylates and inactivates protein phosphatase 2A. The funtion appears to be related to the catalytic domain of p21-activated p65(PAK) protein kinase which is produced in apoptotic cells. AK is also involved in cytoskeleton organization and other signal transduction processes.
Unit Definition
One unit is the amount of enzyme that will incorporate 1 nmole of phosphoryl groups using myelin basic protein as a substrate per minute at pH 7 at 30 deg C.
Physical form
50 ng per vial in 10 μl of 50 mM Tris-HCl, pH 7.0, containing 14 mM β-Mercaptoethanol, 1 mM benzamidine, 0.1 mM PMSF, 1 mM EDTA, 65% ethyleneglycol, and 0.1% Brij® 35.
Legal Information
Brij is a registered trademark of Croda International PLC
Storage Class
10 - Combustible liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Yadong Fan et al.
Frontiers in immunology, 13, 959495-959495 (2022-08-16)
Macrophages are involved in hepatocyte steatosis and necroinflammation and play an important role in the pathogenesis of non-alcoholic fatty liver disease (NAFLD). Impaired autophagy function (decreased autophagy or blocked autophagic flow) leads to cell damage and death and promotes NAFLD
Jinlan Luo et al.
The Journal of biological chemistry, 296, 100667-100667 (2021-04-18)
The epoxyeicosatrienoic acid (EET) exerts beneficial effects on insulin resistance and/or hypertension. EETs could be readily converted to less biological active diols by soluble epoxide hydrolase (sEH). However, whether sEH inhibition can ameliorate the comorbidities of insulin resistance and hypertension
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service