A8811

Aldolase from rabbit muscle

ammonium sulfate suspension, 10-20 units/mg protein

• Synonym(s): D-Fructose-1,6-bisphosphate-D-glyceraldehyde-3-phosphate-lyase, Fructose-diphosphate Aldolase
• CAS Number: 9024-52-6
• Enzyme Commission number: 4.1.2.13 (BRENDA, IUBMB)
• MDL number: MFCD00130453
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: rabbit muscle
• Quality Level: 200
• form: ammonium sulfate suspension
• specific activity: 10-20 units/mg protein
• foreign activity: glyceraldehyde-3-phosphate dehydrogenase ≤0.03%; lactic dehydrogenase ≤0.03%; phosphoglucose isomerase ≤0.6%; pyruvate kinase ≤0.1%; triosephosphate isomerase ≤0.05%
• storage temp.: 2-8°C

Description

General description

Aldolase exists as three isoforms in rabbit, which includes type A from muscle, type B from liver and brain associated type C. Aldolases correspond to a molecular weight of 158 kDa and exists as tetramer.[1]

Application

Aldolase from rabbit muscle has been used:

• in standard 1-phosphofructokinase from rabbit muscle (RPFK-1) assay[2]
• as a standard in the characterization of metabolic enzymes from glaucomatous tissues[3]
• in fructose 2,6-bisphosphate assay of human cell lines[4]

Aldolase is used to convert fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate. Aldolase, from rabbit muscle has been used for stereospecific deprotonation at DHAP C3 [5].

Biochem/physiol Actions

Aldolase interaction with Wiskott-Aldrich syndrome protein (WASP) may modulate actin dynamics.[6] It reverses the inhibition elicited by ascorbate on Muscle-type LDH (LDH-m4).[7]

Aldolase is involved in gluconeogenesis, the Calvin cycle and glycolysis. Aldolase, from rabbit muscle, is a class I aldolase which forms covalent Schiff base intermediates. The active site of aldolase is in the center of the α/β ⁸ barrel fold [5].

Unit Definition

One unit will convert 1.0 μmole of fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate per min at pH 7.4 at 25 °C.

Physical form

Crystalline suspension in 2.5 M (NH₄)₂SO₄, 0.01 M Tris, pH 7.5, 0.001 M EDTA

Analysis Note

Protein determined by biuret.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable