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A8082

Sigma-Aldrich

Antifoam O-30

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About This Item

UNSPSC Code:
12161901

form

emulsion (aqueous)

Quality Level

concentration

100% (organic)

technique(s)

cell culture | hybridoma: suitable
microbiological culture: suitable

density

0.98 g/mL at 25 °C

storage temp.

room temp

suitability

bacteria (fermentation)

Other Notes

A long-lasting fatty acid ester antifoam, 100% active.

Caution

Can be autoclaved repeatedly.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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L A van der Pol et al.
Biotechnology progress, 9(5), 504-509 (1993-09-01)
Addition of high concentrations of silicone antifoam (AF) to a suspension of hybridoma cells in a bubble column reduces the death rate when using medium without a protective component. In contrast, high AF concentrations can cause detrimental effects in a
S Zhang et al.
Journal of biotechnology, 25(3), 289-306 (1992-09-01)
Foam formation and the subsequent cell damage/losses in the foam layer were found to be the major problems affecting cell growth and monoclonal antibody (MAb) production in stirred and sparged bioreactors for both serum-supplemented and serum-free media. Surfactants in the
[The application of antifoam silicones in the microbiological fermentation technic].
L Zalay et al.
Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. 1. Abt. Medizinisch-hygienische Bakteriologie, Virusforschung und Parasitologie. Originale, 197(1), 118-126 (1965-07-01)
J R Hermann et al.
Applied and environmental microbiology, 72(7), 4811-4818 (2006-07-06)
The objective of this research was to optimize sampling parameters for increased recovery and detection of airborne porcine reproductive and respiratory syndrome virus (PRRSV) and swine influenza virus (SIV). Collection media containing antifoams, activated carbons, protectants, and ethylene glycol were

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