A7981

Anti-Aromatase antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-ARO, Anti-CYP XIX, Anti-CYP19A1 (Cytochrome P450, Family 19, Subfamily A, Polypeptide 1), Anti-Estrogen Synthase, Anti-P-450 Aromatase
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 50-55 kDa
• species reactivity: human
• concentration: ~1.0 mg/mL
• technique(s): indirect immunofluorescence: 0.5-1 μg/mL using paraformaldehyde fixed HEK-293T cells transfected with Aromatase; western blot: 0.5-1 μg/mL using human placenta tissue lysate
• UniProt accession no.: P11511
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... CYP19A1(1588)

Description

General description

The enzyme aromatase is a member of P450 cytochrome family. Aromatase catalyzes three oxidation reactions of androstenedione A that lead to aromatization of androgens and subsequent synthesis of estrogen. The enzymatic complex of aromatase is a heterocomplex of cytochrome 450 aromatase and NADPH cytochrome P450 reductase.[1][2] The tissues that produce aromatase are placenta, ovary, brain, bone, adipose tissue, skin and testis.[3] The aromatase enzyme complex is present in the smooth endoplasmic reticulum of cells of these tissues.[4] High levels of aromatase and estrogen are produced by the breast cancer cells. Selective aromatase inhibitors are developed to control the recurrence and survival of hormone-dependent breast cancer tissue[1]
Anti-Aromatase specifically recognizes human aromatase (50-55 kDa).

Application

Anti-Aromatase may be used for immunoblotting at a working concentration of 0.5-1 μg/ml using lysate of human placenta tissue. The antibody has also been used for immunoblotting using whole extracts[5] or nuclear extracts[6] of human primary trophoblasts. For immunofluorescence, a working concentration of 0.5-1 μg/ml is recommended.

Target description

Aromatase is part of the cytochrome p450 family of monooxygenases. It is involved in the production of estrogen and found localized to the endoplasmic reticulum of multiple cell types. [7]

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)