A0847
Adenylyl Cyclase Toxin from Bordetella pertussis
≥70% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, ≥50 units/mg protein
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About This Item
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recombinant
expressed in E. coli
Quality Level
assay
≥70% (SDS-PAGE)
form
lyophilized powder
specific activity
≥50 units/mg protein
mol wt
177 kDa by amino acid sequence
storage temp.
2-8°C
Gene Information
human ... CAMP(820)
mouse ... CAMP(12796)
rat ... CAMP(316010)
Biochem/physiol Actions
Adenylate cyclase toxin from Bordetella pertussis belongs to the family of pore-forming toxins. It converts host ATP to cyclic AMP (cAMP). May be used to affect intracellular cAMP levels in mammalian cells.
Unit Definition
One unit will produce 1.0 μmol cAMP from ATP per minute at pH 8.0 at 30 °C
Reconstitution
When reconstituted with 50 μL of water, the protein is in 0.05 M Tris, 8 M urea, 0.002 M EDTA, pH 8.0.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Certificates of Analysis (COA)
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