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Key Documents

A0847

Sigma-Aldrich

Adenylyl Cyclase Toxin from Bordetella pertussis

≥70% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, ≥50 units/mg protein

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About This Item

MDL number:
UNSPSC Code:
41106305

recombinant

expressed in E. coli

Quality Level

assay

≥70% (SDS-PAGE)

form

lyophilized powder

specific activity

≥50 units/mg protein

mol wt

177 kDa by amino acid sequence

storage temp.

2-8°C

Gene Information

human ... CAMP(820)
mouse ... CAMP(12796)
rat ... CAMP(316010)

Biochem/physiol Actions

Adenylate cyclase toxin from Bordetella pertussis belongs to the family of pore-forming toxins. It converts host ATP to cyclic AMP (cAMP). May be used to affect intracellular cAMP levels in mammalian cells.

Unit Definition

One unit will produce 1.0 μmol cAMP from ATP per minute at pH 8.0 at 30 °C

Reconstitution

When reconstituted with 50 μL of water, the protein is in 0.05 M Tris, 8 M urea, 0.002 M EDTA, pH 8.0.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

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