93443

Triton^™ X-100 solution

BioUltra, for molecular biology, ~10% in H₂O

• Synonym(s): Triton^™ X-100, 4-(1,1,3,3-Tetramethylbutyl)phenyl-polyethylene glycol solution, t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether
• Linear Formula: t-Oct-C₆H₄-(OCH₂CH₂)_xOH, x= 9-10
• CAS Number: 9036-19-5
• Beilstein/REAXYS Number: 2315025
• MDL number: MFCD00128254
• UNSPSC Code: 12161900
• PubChem Substance ID: 57653560
• NACRES: NA.25

Properties

• grade: for molecular biology
• Quality Level: 200
• description: non-ionic
• product line: BioUltra
• form: liquid
• mol wt: micellar avg mol wt 80,000; average mol wt 625
• concentration: ~10% in H₂O
• aggregation number: 100-155
• impurities: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• refractive index: n20/D 1.349±0.002
• pH: 6.5-8.5 (25 °C)
• CMC: 0.2-0.9 mM (20-25°C); 0.23 mM
• transition temp: cloud point 65 °C; pour point ~7 °C
• density: 1.01 g/mL at 20 °C; 1.070 g/cm³
• cation traces: Al: ≤1 mg/kg; Ba: ≤1 mg/kg; Bi: ≤1 mg/kg; Ca: ≤5 mg/kg; Cd: ≤1 mg/kg; Co: ≤1 mg/kg; Cr: ≤1 mg/kg; Cu: ≤1 mg/kg; Fe: ≤1 mg/kg; K: ≤100 mg/kg; Li: ≤1 mg/kg; Mg: ≤1 mg/kg; Mn: ≤1 mg/kg; Mo: ≤1 mg/kg; Na: ≤100 mg/kg; Ni: ≤1 mg/kg; Pb: ≤1 mg/kg; Sr: ≤1 mg/kg; Zn: ≤1 mg/kg
• absorption: cut-off at 310 nm
• SMILES string: CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1
• HLB: 13.5
• InChI: 1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3
• InChI key: HNLXNOZHXNSSPN-UHFFFAOYSA-N

Description

General description

Triton^™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.[1][2]

Application

Triton^™ X-100 has been used:

• for permeabilization of cells before techniques like indirect immunofluorescence and flow cytometry[3][4]
• As a component of lysis buffer used for homogenization of cells[3][4][5]
• As a component of blocking buffer in immunofluorescence staining[6]
• For the preparation of cells in double fluorescence staining[4]

Biochem/physiol Actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

Features and Benefits

• Suitable for Molecular Biology and Biochemical Research
• Free from DNase, RNase, NICKase and protease
• Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications.
• High purity product for research applications
• Tested for the presence of Anionic and Cationic traces

Other Notes

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Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Safety Information

• pictograms: GHS05,GHS09
• signalword: Danger
• hcodes: H315,H318,H410
• pcodes: P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313
• Hazard Classifications: Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2
• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: 483.8 °F - closed cup
• flash_point_c: 251 °C - closed cup
• ppe: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter