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86186

Sigma-Aldrich

Sulforhodamine B acid chloride

suitable for fluorescence, technical

Synonym(s):

Lissamine™ rhodamine B sulfonylchloride

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About This Item

Empirical Formula (Hill Notation):
C27H29ClN2O6S2
CAS Number:
62796-29-6
Molecular Weight:
577.11
Beilstein/REAXYS Number:
7734927
EC Number:
263-735-8
MDL number:
MFCD00042001
UNSPSC Code:
12352106
PubChem Substance ID:
57653085
NACRES:
NA.32

grade

technical

Quality Level

100

form

powder

fluorescence

λex 560 nm; λem 580 nm in chloroform
λex 560 nm; λem ~580 nm in chloroform

suitability

suitable for fluorescence

shipped in

wet ice

storage temp.

−20°C

SMILES string

CCN(CC)c1ccc2c(OC3=C\C(C=CC3=C2c4ccc(cc4S([O-])(=O)=O)S(Cl)(=O)=O)=[N+](/CC)CC)c1

InChI

1S/C27H29ClN2O6S2/c1-5-29(6-2)18-9-12-21-24(15-18)36-25-16-19(30(7-3)8-4)10-13-22(25)27(21)23-14-11-20(37(28,31)32)17-26(23)38(33,34)35/h9-17H,5-8H2,1-4H3

InChI key

YERWMQJEYUIJBO-UHFFFAOYSA-N

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General description

Sulforhodamine B is a fluorescent dye.

Application

Sulforhodamine B acid chloride has been used to synthesize rhodamine-labeled polycation (PLL-ROD). It has also been used in fluorescent protein tracer preparation.
Fluorescent protein label forming stable conjugates

Biochem/physiol Actions

The sulforhodamine B (SRB) assay is useful in determining the density of cells.

Legal Information

Lissamine is a trademark of PerkinElmer Inc.

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A combined numerical and experimental framework for determining permeability properties of the arterial media
Comerford A, et al.
Biomechanics and Modeling in Mechanobiology, 14(2), 297-313 (2015)
J M Kagan
Journal of dental research, 59, 1750-1756 (1980-11-01)
The histopathologic status of gingival tissue was assessed according to the criteria of Page and Schroeder.(5) Rhodamine-labeled bacteria were used to assess the antigenic specificity of plasma cells in the tissue. Examination of tissue sections revealed that 31.6% of plasma
J Rosenecker et al.
Proceedings of the National Academy of Sciences of the United States of America, 93(14), 7236-7241 (1996-07-09)
We have used a pharmacologic mediator to open intercellular connections in selected vessels to allow liposomes to escape from the blood stream and to extravasate into tissues that have appropriate receptors. We have examined the effects of substance P (SP)
J W Sanger et al.
The Journal of cell biology, 99(3), 918-928 (1984-09-01)
To determine if a living cell is necessary for the incorporation of actin, alpha-actinin, and tropomyosin into the cytoskeleton, we have exposed cell models to fluorescently labeled contractile proteins. In this in vitro system, lissamine rhodamine-labeled actin bound to attachment
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