79390
Phosphatase, Alkaline from calf intestinal mucosa
suitable for enzyme immunoassay, solution (clear, colorless), ~2500 U/mg protein (~10 mg/ml)
Synonym(s):
Alkaline Phosphatase from calf intestinal mucosa
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About This Item
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biological source
bovine (calf) intestinal mucosa
form
solution (clear, colorless)
specific activity
~2500 U/mg protein (~10 mg/ml)
mol wt
Mr ~140000
technique(s)
enzyme immunoassay: suitable
impurities
0.1 mM ZnCl2
1 mM MgCl2
3 M NaCl
30 mM triethanolamine
pH
~7.6
storage temp.
2-8°C
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Application
Alkaline phosphatase is used in the laboratory to remove phosphate monoesters which prevents self-ligation. It is also used in the dairy industry to confirm pasturization of milk. Alkaline phosphatse is commonly conjugated to antibodies and used in immunoblotting, ELISA′s, and other antibody based detection assays.
Biochem/physiol Actions
Alkaline phosphatase is a hydrolytic enzyme which dephosphorylates a variety of molecules under basic conditions.
Unit Definition
1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol 4-nitrophenyl phosphate per minute at pH 9.8 and 37 °C
Reconstitution
The solution can be used without dialysis (for enzyme immuno-assay).
Other Notes
Sales restrictions may apply
Used in highly sensitive (pg/m) enzyme immunoassays with LCEC detection of phenol[1]; Comparison of three methods for conjugating alkaline phosphatase to human placental lactogen[2]; Preparation of enzyme-antibody conjugates[3][4]; Liposome immunoassay with antigen-coupled liposomes containing alkaline phosphatase[5]
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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C K Kim et al.
Journal of immunological methods, 159(1-2), 101-106 (1993-02-26)
Immunoliposomes were prepared and their immunoassay applications investigated. Liposomes were prepared from cholesterol and phospholipids including maleimidobenzoylphosphatidylethanolamine (MBPE) for conjunction with thiol-containing antigens. Alkaline phosphatase (APase) was entrapped in the liposome and BSA, the antigen, was modified by reaction with
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Biochemical Society Transactions, 14, 473-473 (1986)
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