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70540

Sigma-Aldrich

Naphthol Yellow S

for microscopy (Hist.)

Synonym(s):

2,4-Dinitro-1-naphthol-7-sulfonic acid sodium salt, 5,7-Dinitro-8-hydroxy-2-naphthalenesulfonic acid sodium salt, Acid Yellow 1, Flavianic acid sodium salt

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$115.00

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25 G
$29.95
100 G
$115.00

About This Item

Empirical Formula (Hill Notation):
C10H4N2Na2O8S
CAS Number:
846-70-8
Molecular Weight:
358.19
Colour Index Number:
10316
Beilstein/REAXYS Number:
3839220
EC Number:
212-690-2
MDL number:
MFCD00003958
UNSPSC Code:
12171500
PubChem Substance ID:
329762555
NACRES:
NA.47

$29.95

List price$36.30Save 17%
Web-Only Promotion

Available to ship onApril 08, 2025Details

Request a Bulk Order

grade

for microscopy (Hist.)

form

powder

solubility

methanol: water (1:1): 0.5 g/10 mL, clear

εmax

≥275 at 423-433 nm in water
≥≥ 250 at 387-397 nm in water

suitability

suitable for microscopy

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].[O-]c1c(cc([N+]([O-])=O)c2ccc(cc12)S([O-])(=O)=O)[N+]([O-])=O

InChI

1S/C10H6N2O8S.2Na/c13-10-7-3-5(21(18,19)20)1-2-6(7)8(11(14)15)4-9(10)12(16)17;;/h1-4,13H,(H,18,19,20);;/q;2*+1/p-2

InChI key

CTIQLGJVGNGFEW-UHFFFAOYSA-L

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Application

Naphthol yellow S has been used as a staining agent in fixed human embryonic stem cell-derived cardiomyocytes.[1]

Biochem/physiol Actions

Naphthol yellow S (NYS) is used as a stain for protein basic groups. It is an acidic dye and forms a NYS-protein complex at acidic pH. Combination of feulgen and NYS provides DNA to protein ratio.[2][3]

Analysis Note

λmax. ∼428 nm/∼392 nm; E(1%,1 cm) ∼275-425/∼250-400 (H2O)

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Warning

hcodes

H317,H373

Hazard Classifications

Skin Sens. 1 - STOT RE 2

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
276745/1

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Scholtissek C, et al.
Chemistry and Cytochemistry of Nucleic Acids and Nuclear Proteins (2012)
J Gaub
The Histochemical journal, 13(5), 717-722 (1981-09-01)
Owing to the accumulation of nuclear non-histone protein (NHP) (a) in cells entering the cell cycle from the quiescent state and (b) in continuously cycling cells during G1 phase, a simultaneous determination of DNA and nuclear NHP is of high
S Gutschmidt et al.
Histochemistry, 72(3), 467-479 (1981-01-01)
In order to quantify the amount of protein in the small intestinal brush border region at different villus sites, cryostat sections of adult rat jejunum were stained with Naphthol Yellow S, Dinitrofluorobenzene and Coomassie Brilliant Blue and the dye deposits
P Skehan et al.
In vitro cellular & developmental biology : journal of the Tissue Culture Association, 21(5), 288-290 (1985-05-01)
A rapid method has been developed for measuring the cellular protein content of mono- and multilayered anchorage cultures. Fixed or air dried cultures are stained for 30 min with 0.2% Naphthol Yellow S (NYS) dissolved in 1% acetic acid. Unbound
M S Huang et al.
Chest, 88(6), 864-869 (1985-12-01)
In order to study differences in the tumor growth and frequency of metastases between younger and older patients with lung cancer, we investigated the nuclear DNA and nuclear protein according to age by means of cytophotometry after combined staining with
View All Related Papers

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