66540386
UOXFi001-C
Human iPS Cell Line
Synonym(s):
EBiSC iPSC Line, Human iPSC, Induced Pluripotent Stem Cell Line, iPS Cell, iPSC
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About This Item
UNSPSC Code:
41106509
biological source
human dermis (fibroblast)
Quality Level
reprogramming method
retrovirus
description
age (70-74)
manufacturer/tradename
EBiSC™
gender
female
growth mode
adherent (pluripotent)
technique(s)
cell culture | stem cell: suitable
relevant disease(s)
Parkinson′s disease
shipped in
dry ice
storage temp.
−196°C
General description
Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.
The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.
Cell Line Origin
Depositor
University of Oxford
University of Oxford
Cell Line Description
Derivation
Primary cell type: Fibroblast of dermis
Tissue collection date: May 3, 2011
Passage number reprogrammed: 4
Reprogramming method
Vector type: Integrating
Vector: Virus
Virus type: Retrovirus
Gene list:
KLF4
MYC
NANOG
POU5F1
SOX2
Absence of reprogramming vector(s): yes
Have the reprogramming vectors been silenced: Unknown
Other:From the same donor: UOXFi001-A, UOXFi001-B, UOXFi001-D
Characterization
Analysis of Undifferentiated Cells
Marker expression: TRA 1-60(+)
PluriTest: Pluripotency score: 23.29
Novelty score: 1.64
Report: UOXFi001-C.PNG
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Genotyping
Karyotyping
Passage number: 13
Cell line karyotype: 46, XX
Karyotyping method: Illumina Human CytoSNP-12v2.1 bead chip array
Primary cell type: Fibroblast of dermis
Tissue collection date: May 3, 2011
Passage number reprogrammed: 4
Reprogramming method
Vector type: Integrating
Vector: Virus
Virus type: Retrovirus
Gene list:
KLF4
MYC
NANOG
POU5F1
SOX2
Absence of reprogramming vector(s): yes
Have the reprogramming vectors been silenced: Unknown
Other:From the same donor: UOXFi001-A, UOXFi001-B, UOXFi001-D
Characterization
Analysis of Undifferentiated Cells
Marker expression: TRA 1-60(+)
PluriTest: Pluripotency score: 23.29
Novelty score: 1.64
Report: UOXFi001-C.PNG
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Genotyping
Karyotyping
Passage number: 13
Cell line karyotype: 46, XX
Karyotyping method: Illumina Human CytoSNP-12v2.1 bead chip array
Linkage
Note: EAUA and CLIP must be completed before order fulfillment
Subculture Routine
Surface coating: Matrigel®/Geltrex®
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: mTeSR® 1
Passage Number banked (pre-EBiSC): 9
No. Vials banked (pre-EBiSC): 30
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: mTeSR® 1
Passage Number banked (pre-EBiSC): 9
No. Vials banked (pre-EBiSC): 30
Legal Information
EBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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