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53892

Sigma-Aldrich

4-Hydrazino-7-nitro-benzofurazan hydrazine adduct

suitable for fluorescence

Synonym(s):

7-Nitro-4-benzofurazanyl-hydrazine hydrazine adduct, NBD-H

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About This Item

Empirical Formula (Hill Notation):
C6H5N5O3 · NH2NH2
CAS Number:
131467-87-3
Molecular Weight:
227.18
MDL number:
MFCD00191508
UNSPSC Code:
12352200
PubChem Substance ID:
329758015

fluorescence

λex 445 nm; λem 522 nm in chloroform (after derivatization with formaldehyde)

suitability

suitable for fluorescence

storage temp.

2-8°C

SMILES string

NN.NNc1ccc([N+]([O-])=O)c2nonc12

InChI

1S/C6H5N5O3.H4N2/c7-8-3-1-2-4(11(12)13)6-5(3)9-14-10-6;1-2/h1-2,8H,7H2;1-2H2

InChI key

BLIOAKMSUDUCKB-UHFFFAOYSA-N

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Application

4-Hydrazino-7-nitro-benzofurazan (HBD) is used as a fluorescent reagent for protein labeling and pre-column derivatization for chromatographic procedures such as HPLC.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Fluorometric detection of aliphatic aldehydes[1]

pictograms

Flame
GHS02

signalword

Warning

hcodes

H228

pcodes

P210

Hazard Classifications

Flam. Sol. 2

Storage Class

4.1B - Flammable solid hazardous materials

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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