51456

Urea

BioUltra, for molecular biology, 99% (T)

• Synonym(s): Carbamide, Carbonyldiamide
• Linear Formula: NH₂CONH₂
• CAS Number: 57-13-6
• Molecular Weight: 60.06
• Beilstein/REAXYS Number: 635724
• EC Number: 200-315-5
• MDL number: MFCD00008022
• UNSPSC Code: 12352111
• PubChem Substance ID: 57651307
• NACRES: NA.25
• grade: for molecular biology
• assay: 99% (T)

Properties

• grade: for molecular biology
• Quality Level: 200
• product line: BioUltra
• assay: 99% (T)
• form: powder or crystals
• impurities: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected; ≤0.1% biuret; <0.005% formaldehyde (HCHO)
• ign. residue: ≤0.01% (as SO₄)
• loss: ≤0.1% loss on drying, 20 °C (HV)
• pH: 8.0-10.0 (20 °C, 8 M in H₂O)
• mp: 132-135 °C (lit.)
• solubility: H₂O: 8 M at 20 °C, clear, colorless
• density: 1.335 g/mL at 25 °C (lit.)
• anion traces: chloride (Cl^-): ≤5 mg/kg; sulfate (SO₄^2-): ≤10 mg/kg
• cation traces: Al: ≤5 mg/kg; As: ≤0.1 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Na: ≤50 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤5 mg/kg; Zn: ≤5 mg/kg
• SMILES string: NC(N)=O
• λ: 8 M in H₂O
• UV absorption: λ: 260 nm A_max: ≤0.03; λ: 280 nm A_max: ≤0.02
• InChI: 1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)
• InChI key: XSQUKJJJFZCRTK-UHFFFAOYSA-N
• Gene Information: human ... CA1(759) , CA2(760) ; rat ... Ppm1a(24666)

Description

Application

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Other Notes

Denaturing reagent[1]; Commonly used in conjunction with polyacrylamide gel electrophoresis for the fractionation of single-stranded nucleic acids with chains of up to 200 nucleotides[2][3][4]

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)