50556
Phalloidin–Atto 390
BioReagent, suitable for fluorescence, ≥90% (HPLC)
Synonym(s):
Atto 390–Phalloidin
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About This Item
UNSPSC Code:
12352116
NACRES:
NA.32
Recommended Products
product line
BioReagent
assay
≥90% (HPLC)
manufacturer/tradename
ATTO-TEC GmbH
λ
in methanol
UV absorption
λ: 382-388 nm Amax
suitability
suitable for fluorescence
storage temp.
−20°C
General description
Atto 390 Phalloidin is a novel fluorescent label with a coumarin structure. The dye is intended for application in the area of life science, e.g. labeling of DNA, RNA or proteins. Characteristic features of the label are high fluorescence quantum yield, large Stokes-shift, good photostability and low molecular weight.Phalloidin is a fungal toxin isolated from the poisonous mushroom Amanita phalloides. Its toxicity is attributed to the ability to bind F actin in liver and muscle cells. As a result of binding phalloidin, actin filaments become strongly stabilized. Phalloidin has been found to bind only to polymeric and oligomeric forms of actin, and not to monomeric actin. The dissociation constant of the actin-phalloidin complex has been determined to be on the order of 3 x 10–8. Phalloidin differs from amanitin in rapidity of action; at high dose levels, death of mice or rats occurs within 1 or 2 hours.
Application
Fluorescent conjugates of phalloidin, rhodamine-phalloidin staining reagents, such as Phalloidin–Atto 390 are used to label actin filaments for histological applications. Some structural features of phalloidin are required for the binding to actin. However, the side chain of amino acid 7 (g-dihydroxyleucine) is accessible for chemical modifications without appreciable loss of affinity for actin.
Legal Information
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Articles
Atto dyes are a series of fluorescent dyes that meet the critical needs of modern fluorescent technologies.
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