Contains sufficient reaction solutions, test mixture and iTRAQ® Reagents 114, 115, 116 and 117 (for 25 μg protein) to label 10 duplex, 6 three-plex or 5 four-plex experiments.
Application
This iTRAQ application kit for protein provides iTRAQ reagents and protocol to label protein prior to digestion. This iChemistry kit can be used in workflows that include SDS-PAGE separation and in-gel protein digestion, followed by LC/MS/MS analysis. Trypsin is sold separately.
Analysis Note
To view the Protocol for the iTRAQ Reagents 4-plex Application Kit - Protein, please visit this Protocol link.
Analysis of the sequence and nature of protein N termini has many applications. Defining the termini of proteins for proteome annotation in the Human Proteome Project is of increasing importance. Terminomics analysis of protease cleavage sites in degradomics for substrate
Cisplatin chemoresistance is a clinical problem that leads to treatment failure in various human epithelial cancers. Members of tumor protein (TP) p53 family play various critical roles in the multiple molecular mechanisms underlying the chemoresistance of tumor cells. However, the
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