27815

Brilliant Blue G

250, for microscopy

• Synonym(s): Acid blue 90, Coomassie Brilliant Blue G
• Empirical Formula (Hill Notation): C₄₇H₄₈N₃NaO₇S₂
• CAS Number: 6104-58-1
• Molecular Weight: 854.02
• Colour Index Number: 42655
• Beilstein/REAXYS Number: 5230822
• EC Number: 228-058-4
• MDL number: MFCD00078482
• UNSPSC Code: 12171500
• PubChem Substance ID: 329752898
• NACRES: NA.26

Properties

• grade: for microscopy
• Quality Level: 200
• form: solid
• SMILES string: [Na+].CCOc1ccc(Nc2ccc(cc2)C(\c3ccc(cc3C)N(CC)Cc4cccc(c4)S([O-])(=O)=O)=C5\C=CC(\C=C5C)=[N+](\CC)Cc6cccc(c6)S([O-])(=O)=O)cc1
• InChI: 1S/C47H49N3O7S2.Na/c1-6-49(31-35-11-9-13-43(29-35)58(51,52)53)40-21-25-45(33(4)27-40)47(37-15-17-38(18-16-37)48-39-19-23-42(24-20-39)57-8-3)46-26-22-41(28-34(46)5)50(7-2)32-36-12-10-14-44(30-36)59(54,55)56;/h9-30H,6-8,31-32H2,1-5H3,(H2,51,52,53,54,55,56);/q;+1/p-1
• InChI key: RWVGQQGBQSJDQV-UHFFFAOYSA-M

Description

General description

Brilliant Blue G (BBG) is a dye. It has been proposed as a substitute for internal limiting membrane (ILM) peeling.

Application

Brilliant Blue G was used to prepare the protein reagent for the determination of protein content of the collagenase enzyme isolated from fish waste. It may be employed as stain for the internal limiting membrane (ILM) for the macular hole (MH) and epiretinal membrane (ERM) surgery.

Biochem/physiol Actions

P2X7 purinergic receptor antagonist.

Analysis Note

λ_max. ∼610 nm; E(1%,1 cm) ∼500-700 (H₂O)

Other Notes

Protein stain for gel electrophoresis. Does not penetrate the gel and is three times more sensitive than Naphthol Blue Black B; Simple and sensitive protein assay; Rapid, sensitive and versatile assay for proteins; free amino acids and several chemicals that interfere with the Lowry procedure cause no interference.

Legal Information

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)