RDD028

Ethanolamine hydrochloride

anhydrous, free-flowing, Redi-Dri^™, ≥99.0%

• Synonym(s): Colamine hydrochloride, Ethanolamine chloride, 2-Aminoethanol hydrochloride
• Linear Formula: H₂NCH₂CH₂OH · HCl
• CAS Number: 2002-24-6
• Molecular Weight: 97.54
• Beilstein/REAXYS Number: 3542892
• MDL number: MFCD00012892
• UNSPSC Code: 12352116
• NACRES: NA.21

Properties

• grade: anhydrous
• Quality Level: 100
• product line: Redi-Dri^™
• assay: ≥99.0%
• quality: free-flowing
• pKa (25 °C): 9.5
• mp: 82-84 °C (lit.)
• SMILES string: NCCO.[H]Cl
• InChI: 1S/C2H7NO.ClH/c3-1-2-4;/h4H,1-3H2;1H
• InChI key: PMUNIMVZCACZBB-UHFFFAOYSA-N

Description

General description

Ethanolamine hydrochloride, a versatile compound in biochemistry and molecular biology, plays a pivotal role in diverse research areas. Primarily, it functions as a buffering agent, ensuring a stable pH environment crucial for enzymatic reactions, protein solubilization, and other biological processes. Its significance extends to membrane protein studies, aiding in the extraction of these proteins from cell membranes. It also finds utility in cell culture and as a reagent for biological sample preparation, simplifying nucleic acid and protein isolation, as well as facilitating the detection of proteins by western blotting and immunohistochemistry. This compound′s wide-ranging applications make it indispensable in biochemical research, contributing to various experimental procedures and advancing our understanding in fields such as molecular biology, immunology, and cellular biology.

Application

Redi-Dri^™ is an innovative packaging product line that helps eliminate clumping material by adding extra packaging process steps without any chemical additives, anti-clumping agents, or hydrophobic compounds. Redi-Dri^™ offers a more convenient and safer way to handle the products.

Ethanolamine hydrochloride has been used:

• in overnight incubation of the tips to attach primary amine groups at the tip surface
• in the preparation of DMEM (dulbecco′s modified eagle′s medium)/F-12 media to culture human epidermal growth factor receptor 2 (HER2) cells derived from MMTV-HER2 transgenic mouse mammary tumors
• to administer the cultures to study its effect on the endogenous phosphatidyl ethanolamine pool and autophagy process

Biochem/physiol Actions

Ethanolamine hydrochloride is utilized as a carbon and nitrogen source by bacteria that differs phylogenetically. It exists as phosphotidylethanolamine in the bacterial and mammalian cell membrane. Ethanolamine-ammonia lyase is responsible for the degradation of ethanolamine into acetaldehyde and ammonia. Ethanolamine is known to positively support lipid accumulation in photosynthetic organism model.

Features and Benefits

• Suitable for molecular biology
• Redi-Dri packaging controls moisture for hygroscopic chemicals that commonly clump
• Powder remains free-flowing and easy to use
• Safer to handle and weigh out for routine use in the lab
• Eliminates clumps, maintaining product moisture, quality, and product specifications

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable