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93349

Sigma-Aldrich

Trizma® base

BioUltra, ≥99.8% (T)

Synonym(s):

2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol

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About This Item

Linear Formula:
NH2C(CH2OH)3
CAS Number:
77-86-1
Molecular Weight:
121.14
Beilstein/REAXYS Number:
741883
EC Number:
201-064-4
MDL number:
MFCD00004679
UNSPSC Code:
12352104
PubChem Substance ID:
329770228

description

aminopeptidase substrate

product line

BioUltra

assay

≥99.8% (T)

impurities

insoluble matter, passes filter test

ign. residue

≤0.01% (as SO4)

loss

≤0.5% loss on drying, 110 °C

pH

10.5-12.0 (25 °C, 4 M in H2O)

useful pH range

7-9

pKa (25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.)
168-172 °C

solubility

H2O: 4 M at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤5 mg/kg
sulfate (SO42-): ≤5 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

4 M in H2O

UV absorption

λ: 260 nm Amax: 0.10
λ: 280 nm Amax: 0.08

SMILES string

NC(CO)(CO)CO

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

InChI key

LENZDBCJOHFCAS-UHFFFAOYSA-N

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Other Notes

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
This product has been replaced by 93362-SIGMA | Trizma base BioUltra, for molecular biology, ≥99.8% (T)
In a high molar concentration for molecular weight determination of peptides and proteins by gel electrophoresis[1]; Electrophoresis of proteins on cellulose acetate membranes[2]; Calibration of polyacrylamide gel columns for the separation of oligonucleotides by capillary electrophoresis[3]; Determination of trace amounts of fluorine from a single sodium carbonate fusion of small geological sample masses[4].

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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M S Garcia et al.
The Analyst, 116(6), 653-656 (1991-06-01)
A kinetic method for the determination of iodide based on its inhibitory effect on the Pd(III) catalysed reaction between ethylenediaminetetraacetic acid (EDTA)-Co(III) and the hypophosphite ion is described. The reaction was followed spectrophotometrically by measuring the decrease in the absorbance
J Ambler et al.
Clinical chemistry, 26(8), 1221-1223 (1980-07-01)
Two new non-barbiturate buffers have been formulated for serum protein electrophoresis on cellulose acetate membranes. Both buffers give five distrinct fractions and are suitable for all systems, but have been primarily designed to meet the needs of the Gelman "Sepratek"
S P Fling et al.
Analytical biochemistry, 155(1), 83-88 (1986-05-15)
Various buffer systems were examined for their ability to resolve and provide molecular weight determinations of proteins and peptides over a wide size range using electrophoresis in sodium dodecyl sulfate-polyacrylamide gels. Sharp bands and high resolution were achieved in the
A Paulus et al.
Electrophoresis, 11(9), 702-708 (1990-09-01)
Polyacrylamide-filled gel columns are used to separate oligonucleotide samples. For homopolymeric standard samples, plots of migration time versus molecular size are presented over a range of 30-160 bases. With 2.5-4% T and 3.3% C gels, good resolution over the examined
Soham Gupta et al.
Frontiers in immunology, 11, 437-437 (2020-04-01)
The hijacking of cellular function through expression of proteins that interfere with the activity of cellular enzymes and regulatory complexes is a common strategy used by viruses to remodel the cell environment in favor of their own replication and spread.
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