Skip to Content
MilliporeSigma
All Photos(1)

Documents

85469

Millipore

SOC Broth

suitable for microbiology

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
41106204
NACRES:
NA.74

sterility

sterile (Filtered and Aseptic Handled)

Quality Level

form

powder

composition

glucose, 20 mM
magnesium chloride, 10 mM
magnesium sulfate, 10 mM
potassium chloride, 2.5 mM
sodium chloride, 10 mM
tryptone, 20 g/L
yeast extract, 5 g/L

technique(s)

microbiological culture: suitable

final pH

7.0±0.2 (25 °C)

application(s)

food and beverages
life science and biopharma

microbiology

storage temp.

2-8°C

suitability

nonselective for Escherichia coli
nonselective for coliforms

Application

SOC Broth is a sterile solution for the recovery of electroporated Escherichia coli cells.

Preparation Note

Immediately after electroporation add 1 ml sterile SOC Medium to the transformed cells and incubate for 30-60 minutes at 37°C with a moderate shaking .

Other Notes

Transformation of E. coli with plasmids

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves


Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Advanced Bacterial Genetics, Cold Spring Harbor (2007)
F.M. Ausubel et al.
Current Protocols in Molecular Biology (1987)
D Hanahan
Journal of molecular biology, 166(4), 557-580 (1983-06-05)
Factors that affect the probability of genetic transformation of Escherichia coli by plasmids have been evaluated. A set of conditions is described under which about one in every 400 plasmid molecules produces a transformed cell. These conditions include cell growth

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service