Peptone from meat, enzymatic digest is a richsource of peptides and free amino acids. Meat peptone provides nitrogen,vitamins, amino acids, and carbon in microbiological culture media. High sulfurcompound content in meat peptone enables the detection of bacteria (Clostridiaspp., Salmonella spp.) that produce hydrogen sulfide. Meat peptone issuitable for the cultivation of yeast and molds, enterobacteria, andstaphylococci. It also favors the growth of streptococci and the production ofcharacteristic hemolysis in blood agar formulations.
Application
Peptone (from meat) is used as a nutritive substrate in fermentation and culture media. It is suitable for analytical microbiology and industrial fermentation.
Protein expression and purification, 164, 105463-105463 (2019-08-06)
Recombinant expression of human membrane proteins in large quantities remains a major challenge. Expression host is an important variable to screen for high-level production of membrane proteins. Using the green fluorescent protein (GFP) as a reporter, we screened the expression
Strategies for high titre plasmid DNA production
in Escherichia coli DH5a
Kevin O?Mahony
Process. Biochem. (2007)
Proposal for a better integration of bacterial lysis into the
production of plasmid DNA at large scale
Journal of molecular biology, 432(18), 5162-5183 (2020-02-28)
Membrane proteins are generally challenging to work with because of their notorious instability. Protein engineering has been used increasingly to thermostabilize labile membrane proteins such as G-protein-coupled receptors for structural and functional studies in recent years. Two major strategies exist.
Culture media provides a habitat with suitable nutrients, energy sources, and certain environmental conditions for the growth of microorganisms. The components of the culture media range from simple sugars to peptones, salts, antibiotics, and complex indicators.
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