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60634

Sigma-Aldrich

Catalase from Micrococcus lysodeikticus

solution, dark brown

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

bacterial (Micrococcus lysodeikticus)

Quality Level

form

solution

specific activity

65,000-150,000 U/mL

mol wt

Mr ~230000

technique(s)

microbe id | metabolite detection: suitable

color

dark brown

solubility

0.05 M phosphate buffer pH 7.0: soluble, clear, brown (1:10)

Protein ID accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

2-8°C

InChI

1S/C9H10O3/c1-2-12-9(11)7-3-5-8(10)6-4-7/h3-6,10H,2H2,1H3

InChI key

NUVBSKCKDOMJSU-UHFFFAOYSA-N

General description

Research area: Cell Signaling

Catalase (CAT) is an antioxidant enzyme, encoded by the CAT gene family. The enzyme is ubiquitously expressed in most of the aerobic organisms.

Application

Catalase from Micrococcus lysodeikticus has been used to eliminate excess of hydrogen peroxide production during sugar analog synthesis by in vitro biocatalytic cascade. It has also been used as a standard to evaluate oxidative stress by indirectly measuring catalytic activity.

Biochem/physiol Actions

Catalase rapidly converts hydrogen peroxide to oxygen and water. It exhibits antioxidant activity and elicits a defense mechanism against reactive oxygen species (ROS). Catalase plays a role in neutralizing noxious hydrogen peroxide in nature as well as in various industries such as textile, pharma, and dairy. Micrococcus lysodeikticus catalase is a heme-containing protein.Catalase, along with various key cellular systems involved in hydrogen peroxide (H2O2) processing, plays a key role in regulating cellular redox balance and aids in cellular and intercellular signaling.

Unit Definition

1 U corresponds to the amount of enzyme which decomposes 1 μmol H2O2 per minute at pH 7.0 and 25 °C

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves


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Margarita Florencio et al.
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Many amphibian species exploit temporary or even ephemeral aquatic habitats for reproduction by maximising larval growth under benign conditions but accelerating development to rapidly undergo metamorphosis when at risk of desiccation from pond drying. Here we determine mechanisms enabling developmental
An improved procedure using ferricyanide for detecting catalase isozymes.
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Analytical biochemistry, 44(1), 301-305 (1971-11-01)
Hessam Sepasi Tehrani et al.
Progress in biophysics and molecular biology, 140, 5-12 (2018-03-14)
Catalase is one of the firsts in every realm of biological sciences. At the same time it also has a number of unusual features. It has one of the highest turnover numbers of all enzymes. It is essential for neutralizing

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