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44715

Sigma-Aldrich

Fast Green FCF

for microscopy

Synonym(s):

Food green 3

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About This Item

Linear Formula:
C37H34N2O10S3Na2
CAS Number:
2353-45-9
Molecular Weight:
808.85
Colour Index Number:
42053
Beilstein/REAXYS Number:
5718212
EC Number:
219-091-5
MDL number:
MFCD00013053
UNSPSC Code:
12171500

grade

for microscopy

mp

290 °C (dec.) (lit.)

SMILES string

[Na+].[Na+].CCN(Cc1cccc(c1)S([O-])(=O)=O)c2ccc(cc2)C(=C3/C=CC(\C=C3)=[N+](\CC)Cc4cccc(c4)S([O-])(=O)=O)\c5ccc(O)cc5S([O-])(=O)=O

InChI

1S/C37H36N2O10S3.2Na/c1-3-38(24-26-7-5-9-33(21-26)50(41,42)43)30-15-11-28(12-16-30)37(35-20-19-32(40)23-36(35)52(47,48)49)29-13-17-31(18-14-29)39(4-2)25-27-8-6-10-34(22-27)51(44,45)46;;/h5-23H,3-4,24-25H2,1-2H3,(H3,41,42,43,44,45,46,47,48,49);;/q;2*+1/p-2

InChI key

RZSYLLSAWYUBPE-UHFFFAOYSA-L

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Application

Fast Green FCF dye is used for protein staining in IEF, native PAGE and SDS-PAGE. Fast Green staining is linear over a wider range of protein concentrations than Brilliant Blue R. After electrophoresis, fixing the proteins in the gel is recommended for maximum sensitivity. The gel can then be stained for 2 hr with 0.1% Fast Green FCF in either 30% (v/v) ethanol, 10% (v/v) acetic acid or in 7% acetic acid. The gel can be destained with either 30% (v/v) ethanol, 10% (v/v) acetic acid, or 7% acetic acid. The stained gel can be scanned at 625 nm. The detection sensitivity is approx. 30% that with Brilliant Blue R.

Other Notes

Reagent for staining proteins after PAGE[1]; Especially suitable in isoelectric focusing (does not bind to ampholytes)[2]

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Staining protein in isoelectric focusing gels with fast green.
R E Allen et al.
Analytical biochemistry, 104(2), 494-498 (1980-05-15)
Studies and critique of Amido Black 10B, Coomassie Blue R, and Fast Green FCF as stains for proteins after polyacrylamide gel electrophoresis.
C M Wilson
Analytical biochemistry, 96(2), 263-278 (1979-07-15)
Sumiko Tsuji et al.
Journal of chromatography. A, 1101(1-2), 214-221 (2005-10-26)
The existence of five of the six expected isomers in commercial Fast Green FCF (G3: Food Green No. 3, FD&C Green No. 3, CAS No. 2353-45-9, C.I. No. 42053), the main product of which is m,m-G3 and the sub-products of
A V Grigor'eva et al.
Tsitologiia, 27(2), 191-195 (1985-02-01)
A cytophotometrical study was made of Fast Green FCF binding by nuclear non-histone proteins of the rat superior ganglion neurons at pH 2.6 (1 day, 1 week, 1 and 5 months, and 2.5 years after birth, a. b.). An increased
R Moss et al.
Stain technology, 57(1), 1-4 (1982-01-01)
It has been shown that gluten powder stained for one minute and forty-five seconds in a 0.192% solution of fast green FCF and then washed repeatedly with distilled water can be detected both by visible light and fluorescence microscopy when
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