agriculture clinical testing environmental food and beverages microbiology
storage temp.
2-8°C
suitability
selective and differential for Escherichia coli selective and differential for coliforms selective and differential for enterovirulent E. coli (EEC)
Application
EC O157 ChromoSelect Agar is a chromogenic medium for isolation and differentiation of E. coli O157 from food and environmental samples.
Preparation Note
Suspend 28.85 g in 1 liter distilled water. Heat to boiling to dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 50°C and pour into sterile petri plates. This medium can be made more selective by aseptically adding 0.25 ml of 1% potassium tellurite solution (Cat. No. 17774) to 1 liter molten and cooled medium (45°C).
Footnote
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Certificates of Analysis (COA)
Lot/Batch Number
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Journal of medical microbiology, 39(2), 155-158 (1993-08-01)
Potassium tellurite was assessed for the selection of verocytotoxigenic (VT+) Escherichia coli O157. MICs were higher for VT+ E. coli O157 than for other strains of E. coli and for Aeromonas spp. MacConkey medium containing sorbitol, tellurite and cefixime (TC-SMAC)
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