If you are checking for the adequate cleaning of equipment, there are various ways to do so. pH indicators such as bromothymol blue can be used to ensure that alkaline detergents have been washed away. However, dyes are generally not commonly used to check for sterility or whether a surface is clean of all substances. The use of other dyes may vary, and it's essential to consider the specific use and characteristics of each dye for different applications.
318752
Bromothymol Blue sodium salt solution
0.04 wt. % in H2O
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About This Item
Empirical Formula (Hill Notation):
C27H27Br2NaO5S
CAS Number:
Molecular Weight:
646.36
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47
Recommended Products
form
liquid
Quality Level
concentration
0.04 wt. % in H2O
pH
6.0-7.6, yellow to blue
density
0.99 g/mL at 25 °C (lit.)
λmax
615 nm
ε (extinction coefficient)
≥13000 at 304-310 nm in 0.1 M NaOH at 0.005 g/L
≥8000 at 391-397 nm in 0.1 M NaOH at 0.005 g/L
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
[Na+].CC(C)c1cc(c(C)c(Br)c1O)\C(=C2/C=C(C(C)C)C(=O)C(Br)=C2C)c3ccccc3S([O-])(=O)=O
InChI
1S/C27H28Br2O5S.Na/c1-13(2)18-11-20(15(5)24(28)26(18)30)23(17-9-7-8-10-22(17)35(32,33)34)21-12-19(14(3)4)27(31)25(29)16(21)6;/h7-14,30H,1-6H3,(H,32,33,34);/q;+1/p-1/b23-21+;
InChI key
NMKFVGALBGZKGW-FKWCIMQXSA-M
Related Categories
General description
Bromothymol Blue is a sulfonated hydroxyquinone in acidic solutions. In alkaline solutions, it is present as a dianion that is blue with a larger conjugated system than the yellow hydroxyquinone. Both forms are lipophilic. It is also known as bromthymol blue or dibromothymolsulfonphthalein.
Application
Bromothymol blue has various applications in biology and medicine. It is suitable for demonstrating fungal hyphae within plant roots. It is used as a vital stain to trace the movement of fluids from the lymph. It is added to an agar gel microbial culture medium for enumeration of Bacillus cereus. Bromothymol blue is also used as a pH indicator due to its precise color transition properties at neutrality.
Biochem/physiol Actions
Bromothymol Blue is a pH indicator which changes color from yellow (pH 6.0) to blue (7.6). It belongs to the class of sulfonephthalein dyes.[1]
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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R. W. Sabnis
Handbook of Acid-Base Indicators (2007)
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