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LB Agar, Vegitone

suitable for microbiology, NutriSelect® Plus

Synonym(s):

Luria Bertani Agar, Vegitone, Vegitone LB Agar

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About This Item

UNSPSC Code:
41171605
NACRES:
NA.85

sterility

non-sterile

Quality Level

200

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15.0 g/L
sodium chloride, 5.0 g/L
tryptone (vegetable), 10.0 g/L
yeast extract, 5.0 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

7.0±0.2 (25 °C)

application(s)

agriculture
food and beverages
life science and biopharma
pharmaceutical
microbiology

suitability

nonselective for Escherichia coli
nonselective for bacteria (General Media)
nonselective for coliforms

Related Categories

General description

LB Agar, Vegitone may be used as a culture media for the cultivation of Agrobacterium tumefaciens for the production of transgenic Citrus plants via Agrobacterium-mediated transformation.

Application

LB Agar Vegitone is free of animal derived material. It is used for the cultivation and maintenance of recombinant strains of Escherichia coli and may be used for routine cultivation of not particularly fastidious microorganisms.

Preparation Note

Suspend 35.0 g in 1000 ml distilled water. Heat to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Dispense as desired.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCL6695
BCCJ6610
BCCH4301
BCCG2212
BCCD8159

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Citrus transformation using mature tissue explants.
Agrobacterium protocols, 259- 273 (2015)
Helen K Alexander et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(32), 19455-19464 (2020-07-25)
A better understanding of how antibiotic exposure impacts the evolution of resistance in bacterial populations is crucial for designing more sustainable treatment strategies. The conventional approach to this question is to measure the range of concentrations over which resistant strain(s)

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