ENDOLYSS-RO

Endoproteinase Lys-C Sequencing Grade

from Lysobacter enzymogenes

• Synonym(s): lys-c, lysyl endopeptidase, protease
• Enzyme Commission number: 3.4.21.50 (BRENDA, IUBMB)
• UNSPSC Code: 12352204

Properties

• biological source: bacterial (Lysobacter enzymogenes)
• grade: protein sequencing grade
• form: lyophilized
• specific activity: ≥200 units/mg protein (at 25 °C, with Chromozym PL)
• mol wt: 33 kDa by SDS-PAGE (reducing)
• purified by: electrophoresis
• packaging: pkg of 3 × 5 μg (11047825001); pkg of 5 μg (11420429001)
• manufacturer/tradename: Roche
• storage condition: (Keep container tightly closed in a dry and well-ventilated place.)
• technique(s): activity assay: suitable
• color: white
• optimum pH: 8.5-8.8
• pH range: 5-12
• solubility: water: soluble
• suitability: suitable for enzyme test
• UniProt accession no.: Q7M135
• storage temp.: 2-8°C

Description

General description

Endoproteinase Lys-C Sequencing Grade is isolated from Lysobacter enzymogenes as a highly purified and specific protease. Endoproteinase Lys-C from Lysobacter enzymogenes is a member of the peptidase S1 family. It is mostly used for the initial fragmentation of polypeptide chains in protein sequence analysis.

Specificity

This enzyme is a serine protease that specifically hydrolyzes amide, ester, and peptide bonds at the carboxylic side of Lys (in Tris-HCl buffer, pH 7.0 to 9.0).
Heat inactivation: Enzyme can be denatured using heat (5 min at 100 °C) or by TCA.

Application

Use Endoproteinase Lys-C Sequencing Grade  for protein structure analysis and for sequence analysis. It is suitable to digest proteins in solution, in polyacrylamide gels or on blotting membranes.

Preparation Note

Storage conditions (working solution): +2 to +8 °C

Reconstitution

Lyophilized Endoproteinase Lys-C sequencing grade is reconstituted in 50 μl double-dist. water. This results in a buffer concentration of 50 mM Hepes, pH 8.0, 10 mM EDTA and 5 mg/ml raffinose. To avoid autolysis, the incubation temperature should not exceed 37 °C.
Stable in 4 M urea.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 2
• flash_point_f: does not flash
• flash_point_c: does not flash