11051458001

dCTP

=98%, solution, 100 mM, suitable for DNA sequencing, suitable for DNA amplification

• Synonym(s): dCTP, 2′-deoxy-cytidine-5′-triphosphate, deoxy-cytidine-5′-triphosphate, 2′-
• Empirical Formula (Hill Notation): C₉H₁₆N₃O₁₃P₃
• Molecular Weight: 467.16
• UNSPSC Code: 41116100

Properties

• Quality Level: 100
• description: Lithium salt
• assay: 98%
• form: solution
• usage: sufficient for 6,250 standard reactions (PCR)
• packaging: pkg of 250 μL (25 μmol)
• manufacturer/tradename: Roche
• concentration: 100 mM
• technique(s): DNA amplification: suitable; DNA sequencing: suitable
• storage temp.: −20°C

Description

General description

dCTP is a 100 mM clear, colorless solution of the lithium salt (pH 7).
dCTP solution in water (pH 7), 100 mM, lithium salt.

Application

Use dCTP, lithium salt, for DNA sequencing, labeling, and all types of amplification reactions or primer-extension reactions.

Quality

Typical analysis:

• ≥98% dCTP (HPLC), ≤1.5% dCDP (HPLC), ≤0.5% dCMP (HPLC)
• Absorbance determination A₂₅₀/A₂₆₀, A₂₈₀/A₂₆₀, A₂₉₀/A₂₆₀
• DNase activity negative, according to the current Quality Control procedures
• Suitability for PCR

Unit Definition

1 μmol dCTP = 9.6 A₂₇₂ units

Storage and Stability

This product is stable at -15 to -25°C until the expiration date printed on the label.
Note: Avoid repeated freezing and thawing. Store in aliquots.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Our PCR Grade deoxynucleotides are even purer than the lithium salts, to ensure excellent performance in your PCRs. PCR Grade deoxynucleotides contain the sodium salt of the nucleotide in PCR Grade Water.
For your convenience, some of our deoxynucleotides are also available in:

• nucleotide sets (all 4 nucleotides in separate vials)
• ready-to-use nucleotide mixes (premixed solutions of all 4 nucleotides)

They are also vital components of:

• kits (dNTPs, buffer, and polymerase in separate vials)
• ready-to-use mixes [polymerase, nucleotides, and buffer (premixed 2x solutions)]

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: nwg
• flash_point_f: No data available
• flash_point_c: No data available