03359123001

Alkaline Phosphatase recombinant, highly active

EIA Grade, from Pichia Pastoris

• Enzyme Commission number: 3.1.3.1 (BRENDA, IUBMB)
• UNSPSC Code: 12352204
• NACRES: NA.21

Properties

• Quality Level: 100
• assay: ≥95% (HPLC)
• form: buffered aqueous solution (ready-to-use, 20 mg/ml)
• specific activity: ≥7000 units/mg protein (At 37 °C with pNPP and DEA as the substrates)
• packaging: pkg of 10 mg (500 μl)
• manufacturer/tradename: Roche
• concentration: 20 mg/mL (ready-to-use)
• optimum pH: 8.0(Stability); 9.8(Activity)
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Alkaline phosphatase (ALP) is a hydrolase enzyme. It is a highly active form in calf intestinal mucosa.[1][2] The calf intestinal alkaline phosphatase is a dimeric glycoprotein with a molecular weight of about 140kDa.[3]

The recombinant, highly active Alkaline Phosphatase possesses the same amino acid sequence as the native, bovine-sourced Alkaline Phosphatase. No animal-derived components are used in the production process. This eliminates the risk of Bovine Spongiform Encephalopathy (BSE) and other infections caused by animals.
The recombinant enzyme guarantees superior lot-to-lot consistency and completely eliminates the risk of BSE.

Specificity

Alkaline phosphatase catalyzed the hydrolysis of numerous phosphate esters, such as esters of primary and secondary alcohols, saccarides, cyclic alcohols, phenols and amines. Phosphodiesters do not react. The enzyme hydrolyzes inorganic pyrophosphate. The kinetic properties of the enzyme depend on many factors, such as enzyme purity, enzyme concentration in the assay, buffer, pH etc.

Application

Alkaline Phosphatase can be coupled to other proteins via its amino and carbohydrate groups. The enzyme solution can be used directly for conjugation without prior dialysis. For conjugation of AP with IgG, a ration of 2.5:1 is recommended. The application of the conjugated enzyme varies from ELISAs and microarrays to western blot analysis.

Biochem/physiol Actions

Alkaline phosphatase (ALP) is responsible for removing phosphate groups at the 5- and 3- positions from many types of molecules, such as nucleotides, proteins, and alkaloids.[2] It can also modify lipopolysaccharides by dephosphorylation of the lipid A moiety, thereby making it less toxic than the unmodified lipid A.[4]

Specifications

≥7,000 U/mg (+37°C; pNPP; DEA)

Physical form

Solution in 3 M NaCl, pH 7.6, 5 mM magnesium chloride, 0.1 mM zinc chloride, 30 mM triethanolamine.

Preparation Note

Activator: Mg^2-, Co^2- and Mn^2-ions

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: No data available
• flash_point_c: No data available