SCT153
Renilla Luciferase Assay
Flash-type Renilla Luciferase assay designed to yield reliable, linear measurements of Renilla luciferase activity with minimal autoluminescence background and superior sensitivity.
Synonym(s):
Firefly Luciferase Assay, Bioluminescent Assay
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.54
Recommended Products
technique(s)
cell based assay: suitable
detection method
chemiluminescent
shipped in
ambient
General description
Renilla luciferase has been used as a reporter gene for studying gene regulation and function in vitro and in vivo. It commonly is used in multiplex transcriptional reporter assays or as a normalizing transfection control for firefly luciferase assays. The enzyme does not require post-translational modification for its activity and may function as a genetic reporter immediately following translation. Coelenterazine also emits light from enzyme-independent oxidation, a process known as autoluminescence. The autoluminescence is enhanced by superoxide anion and peroxynitrite in cells and tissues.
The Renilla Luciferase Assay utilizes a special buffer formulation designed to yield reliable, linear measurements of Renilla luciferase activity with minimal autoluminescence background and superior sensitivity. This is a flash-type luminescence assay that requires signal to be measured immediately after adding working solution to samples. The luminescence signal decays over the course of about 2 minutes of reaction time, although signal half-life may vary depending on luciferase expression levels.
The Renilla Luciferase Assay utilizes a special buffer formulation designed to yield reliable, linear measurements of Renilla luciferase activity with minimal autoluminescence background and superior sensitivity. This is a flash-type luminescence assay that requires signal to be measured immediately after adding working solution to samples. The luminescence signal decays over the course of about 2 minutes of reaction time, although signal half-life may vary depending on luciferase expression levels.
Application
Firefly Luciferase Assay
Flash-type Renilla Luciferase assay designed to yield reliable, linear measurements of Renilla luciferase activity with minimal autoluminescence background and superior sensitivity.
Research Category
Cell Signaling
Cell Signaling
Research Sub Category
Live Cell Dye
Live Cell Dye
Components
1) 5X Passive Lysis Buffer (CS224582): 30 mL
2) Renilla Luciferase Assay Buffer 2.0 (CS224583): 100 mL
3) Aquaphile Coelenterazine (CS224584): 1 X 4 mg
2) Renilla Luciferase Assay Buffer 2.0 (CS224583): 100 mL
3) Aquaphile Coelenterazine (CS224584): 1 X 4 mg
Physical form
liquid
Storage and Stability
Store Renilla Luciferase Assay at –80°C. Renilla Luciferase Assay Buffer 2.0 is stable at -80°C for at least six months from date of receipt. Other kit components are stable at -20°C for at least six months from date of receipt. Kit components and stock solutions of Aquaphile coelenterazine in water are stable to at least 5 freeze/thaw cycles.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class
10 - Combustible liquids
Certificates of Analysis (COA)
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Articles
Firefly luciferase is a sensitive reporter for gene studies due to its absence in mammalian cells or tissues.
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