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SCT019

Sigma-Aldrich

BioTracker 560 Orange Lysosome Dye

Live cell imaging dye for acidic cellular organelles such as lysosomes.

Synonym(s):

Live cell imaging probe

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10 X 10 μG
$549.00

$549.00

Estimated to ship onMarch 26, 2025

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10 X 10 μG
$549.00

About This Item

UNSPSC Code:
12352207
eCl@ss:
32161000
NACRES:
NA.47

$549.00

Estimated to ship onMarch 26, 2025

technique(s)

cell based assay: suitable

detection method

fluorometric

shipped in

ambient

Related Categories

General description

Lysosomes are membrane-enclosed organelles that contain an array of enzymes capable of breaking down all types of biological material including proteins, nucleic acids, carbohydrates, and lipids. Lysosomes function as the digestive system of the cell, serving both to degrade material taken up from outside the cell and to digest obsolete components of the cell itself.

The BioTracker 560 Orange Lysosome Dye is a live cell fluorescence imaging probe which enhances fluorescence dramatically in acidic environments. This probe can stain acidic organelles such as lysosome, late endosome and granule selectively. Namely, in the condition of pH 5.0, identical to the environment of acidic organelles, fluorescence intensity is 50-fold or more compared to physical pH 7.4. The BioTracker 560 Orange Lysosome Dye shows great photostability against irradiating excitation light. As it emits orange color fluorescent by excitation at 532nm or 514 nm, multicolor imaging is possible by combining with blue fluorescence (CFP, Hoechst, etc.), green fluorescence (GFP, fluorescein, etc.) and near-infrared fluorescence.

Spectral Properties
Absorbance: 535nm
Emission: 560nm

Application

Live cell fluorescent imaging
Research Category
Cell Imaging
Research Sub Category
Live Cell Dye

Physical form

Lyophilized

Storage and Stability

Store BioTracker 560 Orange Lysosome Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Questions

  1. How to reconstitute? Recomm concentrations?

    1 answer

    1. See below for the preparation and usage instructions for this product.

      Preparation of Reagent and Cell Staining
      Before opening the vial, spin down the solid to the bottom by a microcentrifuge or by a desktop centrifuge.
      1. To prepare a stock solution, dissolve the BioTracker 560 Orange Lysosome Dye 10 µg in 13.4 µL of distilled water or DMSO. It is not recommended to store DMSO solution of dye because this dye is not stable in DMSO.
      2. Dilute the stock solution with medium to prepare 1 µM stain solution.
      3. Remove the culture medium from cell culture dish and wash twice with medium.
      Caution : Glass bottom dish etc. are recommended as cell culture dish, because it shows a low autofluorescence.
      4. Add stain solution to the dish and incubate for 1 to 2 hours at 37 ?, 5% CO2. Even though the degree of stained
      cells is varied with cell type or growth condition, usually cells are well stained around 2 hours.
      5. After staining, wash once with medium and twice with HBSS buffer. Replace to HBSS buffer and observe the cells using a fluorescence microscopy.

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