A stock solution was prepared at 5 mM in DMSO as a 5000X concentrated solution from cat# T5648 before adding it to the cell culture medium. The cells are thawed and expanded in the MCF-7/TAMR-1 Expansion Media, which includes DMEM/F12 medium without phenol red (Sigma Cat. No. D6434), 1% FBS (EMD Millipore Cat. No. ES-009-B), 2.5 mM L-Glutamine (EMD Millipore Cat. No. TMS-002-C), 6 ng/mL insulin (Sigma Cat. No. I-9278), and 1 uM tamoxifen (Sigma Cat. No. T5648).
SCC101
MCF-7/TAMR-1 Human Breast Cancer Cell Line
Human
Synonym(s):
MCF7 TAMR-1
Human breast cancer cell line
Tamoxifen resistant breast cancer cell line, MCF7 TAMR-1 Human breast cancer cell line Tamoxifen resistant breast cancer cell line
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About This Item
UNSPSC Code:
41106514
eCl@ss:
32011203
NACRES:
NA.81
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Product Name
MCF-7/TAMR-1 Human Breast Cancer Cell Line, MCF-7/TAMR-1 is a tamoxifen-resistant cell line derived from the MCF-7/S0.5 human breast cancer cell line by long term treatment with the drug tamoxifen.
biological source
human
Quality Level
technique(s)
cell culture | mammalian: suitable
shipped in
ambient
General description
Estrogen receptor-positive breast cancer is the most common type of breast cancer. About 80% of all breast cancers express the estrogen receptor (ER+) and depend on estrogen to support the growth and spread of the cancer cells. Hormonal therapy, such as tamoxifen, may help slow or stop the growth of ER+ breast cancers by blocking the actions of estrogen. Primary or acquired tamoxifen resistance poses a major clinical challenge in the treatment of ER+ breast cancer. The MCF-7/TAMR-1 cell line provides a model cell system for studying tamoxifen resistance, elucidating signaling pathways involved in tamoxifen-resistant growth, and identifying new predictive markers for response to hormonal therapy.
MCF-7/TAMR-1 is a tamoxifen-resistant cell line derived from the MCF-7/SO.5 cell line that has been adapted to grow at low serum concentration under the long-term treatment with 1 M with tamoxifen. MCF-7/TAMR-1 cells should be maintained in 1 M tamoxifen and may be compared with the parental MCF-7/S0.5 cells, which are sensitive to tamoxifen.
MCF-7/TAMR-1 is a tamoxifen-resistant cell line derived from the MCF-7/SO.5 cell line that has been adapted to grow at low serum concentration under the long-term treatment with 1 M with tamoxifen. MCF-7/TAMR-1 cells should be maintained in 1 M tamoxifen and may be compared with the parental MCF-7/S0.5 cells, which are sensitive to tamoxifen.
Cell Line Description
Cancer Cells
Application
This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact [email protected].
Quality
• Each vial contains ≥ 1X106 viable cells.
• Cells are tested by PCR and are negative for HPV-16, HPV-18, Hepatitis A, B, C and HIV-1 & 2 viruses.
• Cells are negative for mycoplasma contamination.
• Each lot of cells are genotyped by STR analysis to verify the unique identity of the cell line.
• Cells are tested by PCR and are negative for HPV-16, HPV-18, Hepatitis A, B, C and HIV-1 & 2 viruses.
• Cells are negative for mycoplasma contamination.
• Each lot of cells are genotyped by STR analysis to verify the unique identity of the cell line.
Storage and Stability
MCF-7/TAMR-1 cells should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
188.6 °F - closed cup - (Dimethylsulfoxide)
flash_point_c
87 °C - closed cup - (Dimethylsulfoxide)
Certificates of Analysis (COA)
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A E Lykkesfeldt et al.
British journal of cancer, 53(1), 29-35 (1986-01-01)
The human breast cancer cell line MCF-7 requires oestrogen to produce and promote growth of tumours in athymic mice. In vitro, however, MCF-7 cells proliferate rapidly without supply of oestrogen (Briand & Lykkesfeldt, 1984). Oestrogen stimulation of proliferation of MCF-7
P Briand et al.
Cancer research, 44(3), 1114-1119 (1984-03-01)
The human breast cancer cell line MCF-7 has been adapted to long-term growth at 0.5 and 0.05% fetal bovine serum. Free cytoplasmic and filled nuclear estrogen receptors were found in cells grown at 5, 0.5, and 0.05% fetal bovine serum.
M W Madsen et al.
Molecular and cellular endocrinology, 109(2), 197-207 (1995-04-01)
Breast cancer patients with an estrogen receptor (ER) positive tumor can be treated with the anti-estrogen tamoxifen, but development of anti-estrogen resistance is a serious problem. We have analyzed a tamoxifen resistant human breast cancer cell line MCF-7/TAMR-1 for alterations
Yan Zhao et al.
Aging, 13(14), 18498-18514 (2021-07-23)
Breast cancer (BC) is a common malignant tumor in females. The challenge in treating BC is overcoming chemoresistance. Exosome-mediated transfer of miRNAs is a molecule-shuttle in intercellular communication. Thus, we aimed to investigate whether exosomal miRNA-205 could affect chemoresistance and
Melissa A Maczis et al.
Journal of lipid research, 59(12), 2297-2307 (2018-10-14)
In breast cancer, 17β-estradiol (E2) plays critical roles mainly by binding to its canonical receptor, estrogen receptor (ER) α66, and eliciting genomic effects. E2 also triggers rapid, nongenomic responses. E2 activates sphingosine kinase 1 (SphK1), increasing sphingosine-1-phosphate (S1P) that binds
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What is the method used in your lab to dissolve tamoxifen for application with MCF-7/TAMR-1 Human Breast Cancer Cell Line?
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