NA14
Anti-Topoisomerase II (Ab-1) Mouse mAb (SWT3D1)
liquid, clone SWT3D1, Calbiochem®
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About This Item
UNSPSC Code:
12352203
biological source
mouse
Quality Level
antibody product type
primary antibodies
clone
SWT3D1, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human
should not react with
rat, mouse
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
isotype
IgG1
shipped in
wet ice
storage temp.
2-8°C
General description
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3X63Ag8.653 mouse myeloma cells. Recognizes the ~170 kDa topoisomerase II protein.
Recognizes ~170 kDa topoisomerase II protein in HeLa cells and breast carcinoma tissue.
Immunogen
recombinant human topoisomerase IIα
Application
Immunoblotting (5 µg/ml, see application references)
Paraffin Sections (2.5 µg/ml, pressure cooker pre-treatment required)
Immunoprecipitation (not recommended)
Paraffin Sections (2.5 µg/ml, pressure cooker pre-treatment required)
Immunoprecipitation (not recommended)
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Physical form
In 50 mM sodium phosphate buffer, 2% gelatin.
Analysis Note
Negative Control
P388D cells
P388D cells
Positive Control
HeLa cells or breast carcinoma tissue
HeLa cells or breast carcinoma tissue
Other Notes
Has a high affinity for topoisomerase IIα and a low to moderate affinity to topoisomerase IIβ. Antibody should be titrated for optimal results in individual systems.
van der Zee, A.G., et al. 1994. Ann. Oncol.5, 75.
Cummings, J. and Smyth, J.F. 1993. Ann. Oncol.4, 533.
Robinson, R.G., et al. 1993. Hybridoma12, 407.
Anderson, H.J. and Roberge, M. 1992. Cell Biol. Intl. Reports16, 717.
Gasser, S.M., et al. 1992. J. Antonie Van Leeuwenhoek62, 15.
Tan, K.B., et al. 1992. Can. Res.52, 231.
Osheroff, N., et al. 1991. Bioessays13, 269.
Chung, T.D.Y., et al. 1989. Proc. Natl. Acad. Sci. USA86, 9431.
Wang, J.C. 1985. Ann. Rev. Biochem.54, 665.
Cummings, J. and Smyth, J.F. 1993. Ann. Oncol.4, 533.
Robinson, R.G., et al. 1993. Hybridoma12, 407.
Anderson, H.J. and Roberge, M. 1992. Cell Biol. Intl. Reports16, 717.
Gasser, S.M., et al. 1992. J. Antonie Van Leeuwenhoek62, 15.
Tan, K.B., et al. 1992. Can. Res.52, 231.
Osheroff, N., et al. 1991. Bioessays13, 269.
Chung, T.D.Y., et al. 1989. Proc. Natl. Acad. Sci. USA86, 9431.
Wang, J.C. 1985. Ann. Rev. Biochem.54, 665.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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