MABT203

Anti-Abl (c-, v-, Bcr-) Antibody, clone 24-21

clone 24-21, from mouse

• Synonym(s): Tyrosine-protein kinase ABL1, Abelson murine leukemia viral oncogene homolog 1, Abelson tyrosine-protein kinase 1, Proto-oncogene c-Abl, p150
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 24-21, monoclonal
• species reactivity: human
• technique(s): immunocytochemistry: suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_005148
• UniProt accession no.: P00519
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... ABI1(10006)

Description

General description

c-Abl, v-Abl, and Bcr-abl are ubiquitously expressed non-receptor tyrosine kinases that are involved in a plethora of cellular processes. c-Abl is involved in cytoskeleton remodeling and facilitates cell adhesion and cell motility. c-Abl achieves cytoskeleton remodeling by phosphorylating and activating a wide variety of cytoskeleton-associated proteins such as WASF3, ANXA1, DBN1, DBNL, CTTN, RAPH1, ENAH, MAPT, and PXN. Abl-1 also phosphorylates a number of receptor tyrosine kinases and is involved in regulating levels of EGFR by endocytosis. c-Abl may also play a role in DNA damage response via the ATM pathway, among other cellular processes. Whereas v-Abl is known to be involved in pre-B cell transformation in mice, Bcr-Abl is reported to promote cellular growth in leukemia cells and maintain CML phenotype. Many v-Abl- and Bcr-abl-mediated signaling events have been described; however the complexity and function of these pathways require further elucidation.

Specificity

This antibody recognizes the c-terminus of c-Abl, v-Abl, and Bcr-Abl.

Immunogen

Recombinant protein corresponding to the carboxyl region of human v-Abl fused with TrpE.

Application

Anti-Abl (c-, v-, Bcr-) Antibody, clone 24-21 detects level of Abl (c-, v-, Bcr-), clone 24-21 & has been published & validated for use in Western Blotting, ICC.

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected Abl (c-, v-, Bcr-) in COS-7 and 7C411 cells (Goga, A., et al. (1993). Mol Cell Biol. 13(8):4967-49775.).

Quality

Evaluated by Western Blot in K562 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Abl (c-, v-, Bcr-) in 10 µg of K562 cell lysate.

Target description

~125 kDa and ~190 kDa observed. Uniprot describes a molecular weight of c-Abl at ~122 kDa Uniprot describes that this protein is subject to post-translational modification. This antibody detected c-Abl (~125 kDa) and Bcr-Abl (~190 kDa) in K562 cell lysate. An uncharacterized band at ~27 kDa may be observed in some cell lysates.

Physical form

Format: Purified

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable