MABS421

Anti-DKK3, clone 4G7 Antibody

ascites fluid, clone 4G7, from mouse

• Synonym(s): Dickkopf-related protein 3, Dickkopf-3, Dkk-3, hDkk-3
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: 4G7, monoclonal
• species reactivity: human
• technique(s): flow cytometry: suitable; western blot: suitable
• isotype: IgG1
• UniProt accession no.: Q9UBP4
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... DKK3(27122)

Description

General description

Dickkopf-related protein 3, also known as Dickkopf-3, or Dkk-3, or hDkk-3, and encoded by the gene DKK3/ REIC is a member of the dickkopf family. DKK3 is a 350 amino acid secreted glycoprotein that is composed of an N-terminal signal peptide and two conserved cysteine-rich domains, which are separated by a 12 amino acid linker region. This secreted protein is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene.

Immunogen

Purified recombinant fragment of human DKK3 expressed in E. Coli

Application

Anti-DKK3, clone 4G7 Antibody is an antibody against DKK3 for use in western blotting & flow cytometry.

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected DKK3 in MCF-7 cells.

Research Category
Signaling

Research Sub Category
Cytoskeletal Signaling

Quality

Evaluated by Western Blotting in HEK293, MCF-7 and HL7702 cell lysates.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected DKK3 in HEK293, MCF-7 and HL7702 cell lysates.

Target description

~44 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Physical form

Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.

Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable