biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
3A9E6, monoclonal
mol wt
calculated mol wt 49.6 kDa
observed mol wt ~54 kDa
purified by
using protein G
species reactivity
human
packaging
antibody small pack of 100 μL
technique(s)
ELISA: suitable
flow cytometry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype
IgG1κ
epitope sequence
Unknown
Protein ID accession no.
UniProt accession no.
shipped in
2-8°C
target post-translational modification
unmodified
Gene Information
human ... CLEC18A(348174)
Related Categories
General description
C-type lectin domain family 18 member A (UniProt: A5D8T8; also known as Mannose receptor-like protein 2, CLEC18A) is encoded by the (also known as MRLP2) gene (Gene ID: 348174) in human. CLEC18A is synthesized with a signal peptide (aa 1-26), which is subsequently cleaved off to generate the mature form (aa 27-446). Its expression is observed in all cells and in peripheral blood cells. It is mainly localized in the endoplasmic reticulum, Golgi apparatus, and endosome. Although it is expressed in normal hepatocytes, it is absent in hepatocellular carcinoma. It binds polysaccharides in a Ca2+-independent manner and shows preferential binding to fucoidal, b-glucans and galactans. It also serves as a co-receptor for TLR3 and contributes to the differential immune responses to poly(I:C) and H5N1 influenza A virus infection. Its levels are shown to be up-regulated upon differentiation of monocytes into macrophages and dendritic cells. Significantly higher levels of CLEC18A are observed subjects with hepatitis C infection and this increased level is associated with increased cryoglobulin levels, which may be related to the occurrence of mixed cryobulinemia (MC) syndrome. (Ref.: Huang, YL., et al. (2021). Nat. Commun. 4;229; Liao, TL., et al. (2018). Sci. Rep. 8; Article 17287; Huang, YL., et al. (2015). J. Biol. Chem. 290(35); 21252-21263).
Specificity
Clone 3A9E6 is a mouse monoclonal antibody that detects human C-type lectin domain family 18 member A (CLEC18A).
Immunogen
Purified recombinant human C-type lectin domain family 18 member A (CLEC18A) Fc fusion protein.
Application
Quality Control Testing
Evaluated by Western Blotting in U937 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected CLEC18A in U937 cell lysate.
Tested Applications
Immunohistochemistry (Paraffin) Analysis: A representative lot detected CLEC18A in Immunohistochemistry applications (Huang, Y.L., et al. (2015). J Biol Chem. 290(35):21252-63).
ELISA Analysis: A representative lot detected CLEC18A in ELISA applications (Liao T.L. et al. (2018). Sci Rep. 8(1):17287).
Western Blotting Analysis: A representative lot detected CLEC18A in Western Blotting applications (Huang, Y.L., et al. (2015). J Biol Chem. 290(35):21252-63; Huang, Y.L, et al. (2021). Commun Biol. 4(1):229).
Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected CLEC18A in human kidney tissue sections.
Flow Cytometry Analysis: A representative lot detected CLEC18A in Immunohistochemistry applications (Huang, Y.L., et al. (2015). J Biol Chem. 290(35):21252-63; Liao T.L. et al. (2018). Sci Rep. 8(1):17287).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in U937 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected CLEC18A in U937 cell lysate.
Tested Applications
Immunohistochemistry (Paraffin) Analysis: A representative lot detected CLEC18A in Immunohistochemistry applications (Huang, Y.L., et al. (2015). J Biol Chem. 290(35):21252-63).
ELISA Analysis: A representative lot detected CLEC18A in ELISA applications (Liao T.L. et al. (2018). Sci Rep. 8(1):17287).
Western Blotting Analysis: A representative lot detected CLEC18A in Western Blotting applications (Huang, Y.L., et al. (2015). J Biol Chem. 290(35):21252-63; Huang, Y.L, et al. (2021). Commun Biol. 4(1):229).
Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected CLEC18A in human kidney tissue sections.
Flow Cytometry Analysis: A representative lot detected CLEC18A in Immunohistochemistry applications (Huang, Y.L., et al. (2015). J Biol Chem. 290(35):21252-63; Liao T.L. et al. (2018). Sci Rep. 8(1):17287).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-CLEC18A, clone 3A9E6, Cat. No. MABS2260, is a mouse monoclonal antibody that detects C-type lectin domain family 18 member A (CLEC18A) and is tested in ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), and Western Blotting.
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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