MABS2199

Anti-Extrahepatic biliary cells Antibody, clone GB5

• Synonym(s): EHBC;Gallbladder cells
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: mouse
• Quality Level: 200
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: GB5, monoclonal
• purified by: using protein G
• species reactivity: human
• packaging: antibody small pack of 100 μg
• technique(s): flow cytometry: suitable; immunofluorescence: suitable; immunohistochemistry: suitable
• isotype: IgG1κ
• epitope sequence: Unknown
• Protein ID accession no.: N/A
• UniProt accession no.: N/A
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

The gallbladder is a small, distensible pouch-like organ that concentrates, and stores bile produced by the liver that helps break down dietary fats. Bile flows out of the liver via hepatic ducts that join with the cystic duct, which conveys bile to and from the gall bladder. Gallbladder is derived from the foregut endoderm during embryonic development. In an un-distended state, its mucosa appears to be thrown into many folds. Its lumen is lined with a high columnar epithelium. This epithelial layer rests on a highly vascularized lamina propria. The biliary epithelium shows a morphological heterogeneity that is strictly associated with a variety of functions performed at the different levels of the biliary tree. These epithelial cells are also involved in absorption of water and inorganic salts and help to concentrate bile. The connective tissue wall of gallbladder contains abundant elastic fibers and layers of smooth muscles that run obliquely. Between the smooth muscle layers and serosa lies a thick layer subserosal layer of connective tissue. The availability of antibodies that preferentially mark cell populations within the extrahepatic biliary system are useful for identification and classification of gallbladder cells in relation to bile physiology, gallstone formation, cell plasticity and renewal, metaplasia, and tumor formation. Clone GB5 is reported to specifically detect a subset of gallbladder mucosal epithelium with basal bias. (Ref.: Galivo, FH., et al. (2015). Stem Cell Res. 15(1); 172-181).

Specificity

Clone GB5 is a mouse monoclonal antibody that detects a subset of mucosal epithelium with basal bias in human gallbladder.

Immunogen

Extrahepatic human biliary cells.

Application

Quality Control Testing

Evaluated by Immunohistochemistry (Paraffin) in human gallbladder tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution of this antibody detected Extrahepatic biliary cells in human gallbladder tissue sections.

Tested applications

Immunohistochemistry Applications: A representative lot detected Extrahepatic biliary cells in Immunohistochemistry applications (Galivo, F.H., et al. (2015). Stem Cell Res.15(1):172-81).

Flow Cytometry Analysis: A representative lot detected Extrahepatic biliary cells in Flow Cytometry applications (Galivo, F.H., et al. (2015). Stem Cell Res. 15(1):172-81).

Immunofluorescence Analysis: A representative lot detected Extrahepatic biliary cells in Immunofluorescence applications (Galivo, F.H., et al. (2015). Stem Cell Res. 15(1):172-81).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Reconstitution

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable