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Key Documents

MABS1694

Sigma-Aldrich

Anti-FABP2 Antibody, clone 4F6.1

clone 4F6.1, from mouse

Synonym(s):

Fatty acid-binding protein, intestinal, Fatty acid-binding protein 2, I-FABP, Intestinal-type fatty acid-binding protein

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

4F6.1, monoclonal

species reactivity

rat, mouse, human

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... FABP2(2169)

General description

Fatty acid-binding protein, intestinal (UniProt P12104; also known as Fatty acid-binding protein 2, I-FABP, Intestinal-type fatty acid-binding protein) is encoded by the FABP2 (also known as FABPI) gene (Gene ID 2169) in human. The intracellular lipid-binding protein (iLBP) multigene family includes 12 fatty acid-binding protein (FABPs), 4 retinol-binding proteins (RBPs) and 2 cellular retinoic acid-binding proteins (CRABPs). FABPs are 14-16 kDa cytosolic proteins that bind fatty acids (FAs) and other hydrophobic ligands, such as eicosanoids and retinoids. FABPs are either numbered by the Hertzel and Bernlohr nomenclature to indicate their chronological order of discovery or named according to the tissue where each FABP was first isolated. In addition to transporting and sequestering long-chain FAs, eicosanoids, bile salts, and other hydrophobic ligands for storage and metabolism, FABPs are long recognized for their role in gene transcription regulation via lipid-mediated activation of nuclear hormone receptors (NHRs). FABP1 and FABP2, for example, are shown to bind a range of PPAR agonists, including long chain fatty acids (LCFA) and drugs. FABPs play differential and ligand-specific roles in NHR activation, and the patterns of drug interaction with intracellular binding proteins such as FABPs may govern tissue-specific actions of drugs that target NHRs.

Specificity

Clone 4F6.1 targets an epitope conserved among human, mouse, and rat species.

Immunogen

GST-tagged recombinant human FABP2.

Application

Detect FABP2 using this mouse monoclonal Anti-FABP2 Antibody, clone 4F6.1, Cat. No. MABS1694, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.
Immunohistochemistry Analysis: A 1:50 dilution from a representative detected FABP2 in human small intestine and rat colon tissue sections.

Western Blotting Analysis: A 1:1,000 dilution from a representative detected FABP2 in 10 µg of human small intestine tissue lysate.
Research Category
Signaling

Quality

Evaluated by Western Blotting in mouse small intestine tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected FABP2 in 10 µg of mouse small intestine tissue lysate.

Target description

~15 kDa observed. 15.11 kDa (human), 14.99 kDa (mouse & rat) calculated (Met1 removed). Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified
Protein G purified.
Purified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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Linda Berková et al.
EMBO reports, 24(9), e56454-e56454 (2023-07-26)
The protective and absorptive functions of the intestinal epithelium rely on differentiated enterocytes in the villi. The differentiation of enterocytes is orchestrated by sub-epithelial mesenchymal cells producing distinct ligands along the villus axis, in particular Bmps and Tgfβ. Here, we

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