MABN504
Anti-VDAC1 Antibody, clone N152B/23
clone N152B/23, from mouse
Synonym(s):
Voltage-dependent anion-selective channel protein 1, VDAC-1, hVDAC1, Outer mitochondrial membrane protein porin 1, Plasmalemmal porin, Porin 31HL, Porin 31HM
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All Photos(3)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
N152B/23, monoclonal
species reactivity
rat, mouse, human
packaging
antibody small pack of 25 μg
technique(s)
immunohistochemistry: suitable
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... VDAC1(7416)
General description
Voltage-dependent anion-selective channel protein 1 (VDAC1, VDAC), also known as outer mitochondrial membrane protein porin 1, is the outer mitochondrial membrane receptor for hexokinase and BCL2L1. VDAC1 forms a channel through the mitochondrial membrane and is involved in small molecule diffusion, cell volume regulation and apoptosis. VDAC1 may participate in the formation of the permeability transition pore complex (PTPC), which is responsible for the release of mitochondrial products that triggers apoptosis.
Specificity
This antibody does not cross-react with VDAC2 or VDAC3 (Prof. J. Trimmer, University of California, Davis).
Immunogen
Recombinant protein corresponding to human VDAC1.
Application
Detect VDAC using this mouse monoclonal antibody, Anti-VDAC1 Antibody, clone N152B/23 validated for use in western blotting & IHC.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Developmental Signaling
Developmental Signaling
Western Blotting Analysis: A representative lot states VDAC1 does not cross-react with VDAC2 or VDAC3 (based on KO validation results) representative lot deteted VDAC1 in wild type mouse liver tissue, and demonstrated a loss of signal in VDAC1 knock out mouse hippocampal tissue using immunobloting analysis (Prof. J. Trimmer, University of California, Davis).
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected VDAC1 in human cardiac myocytes tissue.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected VDAC1 in human cardiac myocytes tissue.
Quality
Evaluated by Western Blotting in mouse brain tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected VDAC1 in 10 µg of mouse brain tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected VDAC1 in 10 µg of mouse brain tissue lysate.
Target description
~ 35 kDa observed
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Mouse brain tissue lysate
Mouse brain tissue lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Product No.
Description
Pricing
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Maria Feola et al.
Communications biology, 4(1), 517-517 (2021-05-05)
Erythropoiesis involves complex interrelated molecular signals influencing cell survival, differentiation, and enucleation. Diseases associated with ineffective erythropoiesis, such as β-thalassemias, exhibit erythroid expansion and defective enucleation. Clear mechanistic determinants of what make erythropoiesis effective are lacking. We previously demonstrated that
Ngonidzashe B Madungwe et al.
American journal of translational research, 12(7), 3412-3428 (2020-08-11)
MPV17 is an inner mitochondrial membrane protein whose mutation results in mitochondrial DNA (mtDNA) depletion diseases such as neurohepatopathy. MPV17 is expressed in several organs including the liver and kidneys. Here, we investigated its role and mechanism of action in
Mitofilin Heterozygote Mice Display an Increase in Myocardial Injury and Inflammation after Ischemia/Reperfusion.
Feng, et al.
Antioxidants (Basel, Switzerland), 12 (2023)
Arnaud Mourier et al.
The Journal of cell biology, 208(4), 429-442 (2015-02-18)
Mitochondria form a dynamic network within the cell as a result of balanced fusion and fission. Despite the established role of mitofusins (MFN1 and MFN2) in mitochondrial fusion, only MFN2 has been associated with metabolic and neurodegenerative diseases, which suggests
Gui-Ying Yao et al.
Cell death & disease, 9(10), 1033-1033 (2018-10-12)
Ischemic postconditioning provides robust neuroprotection, therefore, determining the molecular events may provide promising targets for stroke treatment. Here, we showed that the expression of functional mitochondrial voltage-dependent anion channel proteins (VDAC1, VDAC2, and VDAC3) reduced in rat vulnerable hippocampal CA1
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