MABN483

Anti-IRAP Antibody, Clone 3E1

clone 3E1, from mouse

• Synonym(s): Leucyl-cystinyl aminopeptidase, Cystinyl aminopeptidase, Insulin-regulated membrane aminopeptidase, Insulin-responsive aminopeptidase, IRAP, Oxytocinase, OTase, Placental leucine aminopeptidase, P-LAP, Leucyl-cystinyl aminopeptidase, pregnancy serum form
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 3E1, monoclonal
• species reactivity: human, mouse
• technique(s): immunocytochemistry: suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_005566
• UniProt accession no.: Q9UIQ6
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... LNPEP(4012)

Description

General description

IRAP, also known as Leucyl-cystinyl aminopeptidase, Cystinyl aminopeptidase, Insulin-regulated membrane aminopeptidase, Insulin-responsive aminopeptidase (IRAP), Oxytocinase (OTase), or Placental leucine aminopeptidase (P-LAP) and encoded by the human gene name IRAP/OTASE, is an important aminopeptidase that plays an important role in maintaining homeostasis during pregnancy. IRAP degrades peptide hormones including oxytocin, vasopressin, and angiotensin III; it also appears to play a role in degrading neuronal peptides in the brain such as Met-enkephalin and dynorphin. IRAP also binds angiotensin IV and appears to be the receptor for angiotensin IV in the brain. IRAP is a membrane bound aminopeptidase and is often found within membrane vesicles that together with other proteins like GLUT4 translocates to the plasma membrane in response to insulin or oxytocin. A cleaved fragment of IRAP, the Leucyl-cystinyl aminopeptidase, is found in the blood during pregnancy, low in the first trimester but rising rapidly until birth, whereby it decreases rapidly, thus the secreted form is often used as a pregnancy marker. IRAP is highly expressed in placenta, heart, kidney and small intestine. IRAP is detected at lower levels in neuronal cells in the brain, in skeletal muscle, spleen, liver, testes and colon.

Immunogen

Epitope: N-terminus

Recombinant protein corresponding to the N-terminus of human IRAP.

Application

Anti-IRAP, Clone 3E1 is an antibody against IRAP for use in WB, ICC.

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

Western Blotting Analysis: A representative lot detected IRAP in 3T3-L1 cells treated with or without insulin (Garza, L.A., et al. (2000). JBC. 275(4):2560-2567).
Immunocytochemistry Analysis: A representative lot detected IRAP in 3T3-L1 cells treated with or without insulin (Garza, L.A., et al. (2000). JBC. 275(4):2560-2567).

Quality

Evaluated by Western Blotting in insulin treated NIH-3T3 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected IRAP in 10 µg of insulin treated NIH-3T3 cell lysate.

Target description

~150 kDa observed. The calculated molecular weight of this protein is 117 kDa, but can be observed at ~150 kDa due to high glycosylation. Uncharacterized band(s) may appear in some lysates.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable