MABN378
Anti-Potassium Channel Kv10.1 Antibody, clone 56
clone 56, from mouse
Synonym(s):
Potassium voltage-gated channel subfamily H member 1, Ether-a-go-go potassium channel 1, EAG channel 1, h-eag, hEAG1, Voltage-gated potassium channel subunit Kv10.1, hEag1 Potassium channel, eag1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
56, monoclonal
species reactivity
rat, human
technique(s)
activity assay: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
isotype
IgG2bκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... KCNH1(3756)
General description
Potassium Channel Kv10.1 is also known as Potassium voltage-gated channel subfamily H member 1, Ether-a-go-go potassium channel 1 (EAG channel 1, h-eag, or hEAG1) and Voltage-gated potassium channel subunit Kv10.1. Potassium Channel Kv10.1 is a pore-forming (alpha) subunit of voltage-gated, non-inactivating, delayed rectifier potassium channel. Potassium Channel Kv10.1 mediates IK(NI) current in myoblasts, and can be modulated by cAMP as well as subunit assembly. Potassium Channel Kv10.1 is highly expressed in myoblasts at the onset of fusion and brain tissue. It is not found in normal epithelial cells, but has been detected in HeLa (cervical carcinoma), SH-SY5Y (neuroblastoma) and MCF-7 (epithelial tumor) cells.
Specificity
This antibody recognizes the extracellular, C-terminus domain of Potassium Channel Kv10.1. Additionally, this antibody does not cross react with hEag2 (Gomez-Varela, D., et al. (2007). Cancer Res. 67(15):7343-7349.).
Immunogen
Epitope: Extracellular domain
Recombinant protein corresponding to the extracellular domain of human Potassium Channel Kv10.1.
Application
Detect Kv10.1 using this mouse monoclonal antibody, Anti-Potassium Channel Kv10.1 Antibody, clone 56 validated for use in IHC, Activity Assay & Immunofluorescence.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Potassium Channel Kv10.1 in human thalamus and cerebellum tissues.
Immunocytochemistry Analysis: A representative lot detected Potassium Channel Kv10.1 in transfected CHO-K1 and HEK293 cells (Gomez-Varela, D., et al. (2007). Cancer Res. 67(15):7343-7349.).
Activity Assay Analsyis: A representative lot from an independent laboratory inhibited anchorage-independent cancer cell growth in vitro (Gomez-Varela, D., et al. (2007). Cancer Res. 67(15):7343-7349.; Chen, Y., et al. (2011). PLoS One. 6(5):e19257.).
Activity Assay Analysis: A representative lot from independent laboratories inhibited Potassium Channel Kv10.1 currents in transfected HEK293 cells (Gomez-Varela, D., et al. (2007). Cancer Res. 67(15):7343-7349.).
Activity Assay Analysis: A representative lot from an independent laboratory inhibited cellular proliferation and
migration of certain Acute myeloid leukemia cell lines and primary cultured AML cells in vitro (Agarwal, J. R., et al. (2010). Mol Cancer. 9:18.).
Immunocytochemistry Analysis: A representative lot detected Potassium Channel Kv10.1 in transfected CHO-K1 and HEK293 cells (Gomez-Varela, D., et al. (2007). Cancer Res. 67(15):7343-7349.).
Activity Assay Analsyis: A representative lot from an independent laboratory inhibited anchorage-independent cancer cell growth in vitro (Gomez-Varela, D., et al. (2007). Cancer Res. 67(15):7343-7349.; Chen, Y., et al. (2011). PLoS One. 6(5):e19257.).
Activity Assay Analysis: A representative lot from independent laboratories inhibited Potassium Channel Kv10.1 currents in transfected HEK293 cells (Gomez-Varela, D., et al. (2007). Cancer Res. 67(15):7343-7349.).
Activity Assay Analysis: A representative lot from an independent laboratory inhibited cellular proliferation and
migration of certain Acute myeloid leukemia cell lines and primary cultured AML cells in vitro (Agarwal, J. R., et al. (2010). Mol Cancer. 9:18.).
Research Category
Neuroscience
Neuroscience
Research Sub Category
Signaling Neuroscience
Signaling Neuroscience
Quality
Evaluated by Immunohistochemistry Analysis in rat frontal lobe tissue.
Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Potassium Channel Kv10.1 in rat frontal lobe tissue.
Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Potassium Channel Kv10.1 in rat frontal lobe tissue.
Target description
111 kDa calculated
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Rat brain tissue
Rat brain tissue
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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