MABN2419

Anti-phospho-alpha-synuclein (Ser129) Antibody, clone LS4-1B1

clone LS4-1B1, from mouse

• Synonym(s): Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, NACP
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: LS4-1B1, monoclonal
• species reactivity: human, mouse
• packaging: antibody small pack of 25 μg
• technique(s): immunohistochemistry: suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_000336
• UniProt accession no.: P37840
• target post-translational modification: phosphorylation (pSer129)
• Gene Information: human ... SNCA(6622)

Description

General description

Alpha-synuclein (UniProt: P37840; also known as Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, NACP) is encoded by the SNCA (also known as NACP, PARK1) gene (Gene ID: 6622) in human. Alpha-synuclein is a membrane-bound protein in dopaminergic neurons that is involved in the regulation of dopamine release and transport. It is also reported to induce fibrillization of microtubule-associated protein tau and reduces neuronal responsiveness to various apoptotic stimuli. Aggregation of alpha-synuclein is known to be a causative factor in the development of Parkinson′s disease (PD), which leads to the degeneration of dopaminergic neurons in the substantia nigra (SN) in the midbrain region. Aggregates of alpha-synuclein are present in Lewy bodies. Alpha-synuclein can be phosphorylated, predominantly on serine residues by casein kinase 1. Phosphorylation of Ser129 is reported to be selective and extensive in synucleinopathy lesions and promotes insoluble fibril formation. Mutations in the SNCA gene produce defective alpha-synuclein that induces conformational changes making it more prone to self-aggregation and deposition in Lewy bodies. Mutant forms of alpha-synuclein impair synaptic vesicle formation, elevate cytoplasmic levels of dopamine, and increases superoxide radicals, which lead to oxidative stress and misfolding of alpha-synuclein.

Specificity

Clone LS4-1B1 specifically detects alpha-synuclein phosphorylated on serine 129.

Immunogen

KLH-conjugated linear peptides corresponding to aa 121-137 and 124-134 phosphorylated on Serine 129 from the C-terminal region of human alpha synuclein.

Application

Anti-phospho-alpha-synuclein (Ser129), clone LS4-1B1, Cat. No. MABN2419, is a highly specific mouse monoclonal antibody that targets alpha-synuclein and neurofilament light polypeptide (NFL) phosphorylated on Ser129 and has been tested in Western Blotting and Immunohistochemistry.

Research Category
Neuroscience

Western Blotting Analysis: A representative lot detected phospho-alpha-synuclein (Ser129) in Western Blotting applications (Rutherford, N.J., et. al. (2016). Acta Neuropathol Commun. 4(1):80).

Immunohistochemistry Analysis: A representative lot detected phospho-alpha-synuclein (Ser129) in Immunohistochemistry applications (Rutherford, N.J., et. al. (2016). Acta Neuropathol Commun. 4(1):80).

Quality

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected phospho-alpha-synuclein (Ser129) in mouse brain tissue lysate.

Target description

~70 kDa observed (aggregated); 14.46 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1