biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
IF15-E2, monoclonal
mol wt
calculated mol wt 64.06 kDa
observed mol wt ~58 kDa
species reactivity
human, mouse
packaging
antibody small pack of 100 μL
technique(s)
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype
IgG
UniProt accession no.
shipped in
dry ice
storage temp.
2-8°C
target post-translational modification
unmodified
Related Categories
General description
SHC-transforming protein 3 (UniProt: Q92529; also known as Neuronal Shc, N-Shc, Protein Rai, SHC-transforming protein C, Src homology 2 domain-containing-transforming protein C3, SH2 domain protein C3, Rai) is encoded by the SHC3 (also known as NSHC, SHCC) gene (Gene ID:53358) in human. Rai protein is mainly expressed in the brain and its higher expression is observed in cerebral cortex, frontal and temporal lobes, hippocampus, and occipital pole. Its expression is also reported in the enteric glial cells in the human gut. It serves as a signaling adapter and is involved in the signal transduction pathways of neurotrophin-activated Trk receptors in cortical neurons. Rai also serves as a physiological substrate of the receptor tyrosine kinase Ret and increases Ret-dependent survival via PI3 kinase/Akt pathway activation. It also potentiates MAP kinase signaling. Rai activation also plays a role in long-term potential and is neuroprotective against certain ischemia and oxidative stress. Evolutionarily it correlates well with vertebrate brain development. Its reduced levels are observed in brain of Alzheimer s disease subjects. Two isoforms of Rai have been described (p64 and p52) that are produced by alternative splicing. (Ref.: Triaca, V., et al. (2018). Aging. 10(1); 5-6; Sagi, O., et al. (2015). Ageing Res. Rev. 19; 34-42; Villanacci, V., et al. (2008). Neurogastroenterol. Motil. 20(3); 206-212).
Specificity
Clone IF15-E2 is a mouse monoclonal antibody that detects SHC-transforming protein 3/RAI. It targets an epitope within the CH1 domain from the C-terminal half.
Immunogen
Recombinant fragment corresponding to 164 amino acids CH1 domain of human SHC-transforming protein 3 (Rai).
Application
Quality Control Testing
Evaluated by Western Blotting in Human brain tissue lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Rai in Human brain tissue lysate.
Tested Applications
Immunohistochemistry Applications: A representative lot detected Rai in Immunohistochemistry applications (Villanacci, V., et. al. (2008). Neurogastroenterol Motil. 20(3):206-12).
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Rai in U2OS cells.
Immunohistochemistry (Paraffin) Analysis: A 1:500 dilution from a representative lot detected Rai in human esophagus, stomach, and colon tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in Human brain tissue lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Rai in Human brain tissue lysate.
Tested Applications
Immunohistochemistry Applications: A representative lot detected Rai in Immunohistochemistry applications (Villanacci, V., et. al. (2008). Neurogastroenterol Motil. 20(3):206-12).
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Rai in U2OS cells.
Immunohistochemistry (Paraffin) Analysis: A 1:500 dilution from a representative lot detected Rai in human esophagus, stomach, and colon tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Rai, clone IF15, Cat. No. MABN2286, is a mouse monoclonal antibody that detects SHC-transforming protein 3 (Rai) and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Physical form
Purified mouse monoclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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