MABE1156

Anti-ING4 Antibody, clone BTIM-4

clone BTIM-4, from mouse

• Synonym(s): Inhibitor of growth protein 4, ING4, p29ING4
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: BTIM-4, monoclonal
• species reactivity: mouse, human
• technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_001121054
• UniProt accession no.: Q9UNL4
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... ING4(51147)

Description

General description

Inhibitor of growth protein 4 (UniProt Q9UNL4; also known as Brain my036 protein, Candidate tumor suppressor p33 ING1 homolog, p29ING4) is encoded by the ING4 gene (ORF My036; Gene ID 51147) in human. The inhibitor of growth (ING) family tumor suppressor ING4 is a transcription regulator that exerts its function via chromatin remodeling. ING4 to targets the trimethylated lysine 4 of histone H3 (H3K4me3) via its conserved plant homeodomain (PHD) finger motif and is shown to associate with the HBO1/JADE histone acetyltransferase (HAT) complex. ING4 is shown to play a role in many cancer-related cellular processes, including cell proliferation, apoptosis, migration, angiogenesis, contact inhibition, DNA damage response, and hypoxia. Both gene deletion and reduced ING4 expression are reported in cancers of diverse tissue types, although ING4 knockout by itself does not cause increased spontaneous tumor formation in mice. Immunohistochemistry analysis of breast cancer tissues shows that 34% of tumors expressed undetectable to low levels of ING4. In addition, ING4 downregulation inversely correlates with an increase in NF-κB activation and NF-κB-dependent genes expression in primary breast tumors.

Specificity

Clone BTIM-4 reacts with human and murine ING4, but not human ING1, ING2, or ING5 (Byron, S.A., et al. (2012). PLoS One. 7(10):e46823). Expected to react with human ING4 isoform 1 (ING4_v1) and isoform 8 (deltaEx6A). Reactivity toward human ING4 spliced isoform 2 to 7 has not been tested.

Immunogen

Epitope: N-terminal half

His-tagged recombinant protein corresponding to the N-terminal half of human ING4.

Application

Anti-ING4 Antibody, clone BTIM-4 is an antibody against ING4 for use in Western Blotting, Immunocytochemistry, Immunohistochemistry.

Research Category
Epigenetics & Nuclear Function

Western Blotting Analysis: A representative lot detected endogenous ING4 in MCF10A and T47D cells, as well as down-regulated ING4 in ING4 shRNA-transfected MCF10A cells (Byron, S.A., et al. (2012). PLoS One. 7(10):e46823).
Western Blotting Analysis: A representative lot detected full-length and degradation fragments of exogenously overexpressed human ING4 in HEK-293T, MCF10A, and T47D cells (Byron, S.A., et al. (2012). PLoS One.;7(10):e46823).
Immunocytochemistry Analysis: A representative lot detected both endogenous and exogenously overexpressed ING4 in MCF10A and T47D cells, as well as down-regulated ING4 in ING4 shRNA-transfected MCF10A cells (Byron, S.A., et al. (2012). PLoS One. 7(10):e46823).
Immunohistochemistry Analysis: A representative lot detected lower ING4 immunoreactivity in more advanced breast tumor tissue samples (Byron, S.A., et al. (2012). PLoS One. 7(10):e46823).
Note: For Western blotting application, the use of nuclear extracts will yield a stronger target band than whole cell lysates.

Quality

Evaluated by Western Blotting in MCF-10A breast carcinoma cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected ING4 in 10 µg of MCF-10A human breast epithelial cell lysate.

Target description

~22 kDa observed

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable