MABE1124
Anti-XPA Antibody, clone 1XPA Antibody-1E11
ascites fluid, clone 1XPA-1E11, from mouse
Synonym(s):
DNA repair protein complementing XP-A cells, XPA, Xeroderma pigmentosum group A-complementing protein
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
antibody form
ascites fluid
antibody product type
primary antibodies
clone
1XPA-1E11, monoclonal
species reactivity
human
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... XPA(7507)
General description
DNA repair protein complementing XP-A cells (UniProt P23025; also known as Excision repair-controlling, Mutant xeroderma pigmentosum complementation group A, Xeroderma pigmentosum group A-complementing protein) is encoded by the XPA (also known as XP1, XPAC) gene (Gene ID 7507) in human. DNA lesions caused by UV irradiation, drugs, or other environmental factors are eliminated by two nucleotide excision repair (NER) pathways, Global genome repair (GGR) and transcription-coupled repair (TCR). In GGR, the removal of lesions requires their recognition by the repair factor XPC/HR23b and the subsequent opening of the DNA duplex by transcription factor II human (TFIIH). The resulting single-stranded structure is stabilized by XPA and replication protein A (RPA). XPG is recruited through its interaction with TFIIH on the 3′ side of the lesion and its positioning on the cut site requires RPA. The interaction between XPA and XPB (ERCC1) stimulates the recruitment of ERCC1-XPF on the 5′ side of the DNA lesion. The damaged oligonucleotide can then be removed through the double incision by XPG and ERCC1-XPF endonucleases. In TCR, these factors (except XPC/HR23B) are recruited by the stalled RNA pol II in front of the damage with the help of the CSB and CSA proteins.
Immunogen
Epitope: Near C-terminus
Linear peptide corresponding to human XPA sequence near C-terminus.
Application
Anti-XPA Antibody, clone 1XPA Antibody-1E11 is an antibody against XPA for use in Western Blotting, Immunocytochemistry.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
Nuclear Receptors
Western Blotting Analysis: A representative lot detected the ATP-dependent recruitment of XPA in HeLa nuclear extract onto the stalled RNA pol IIO on mono-cisplatin-damaged DNA (Riedl, T., et al. (2003). EMBO J. 22(19):5293-5303; Laine, J.P., and, Egly, J.M. (2006). EMBO J. 25(2):3873-97).
Immunocytochemistry Analysis: A representative lot detected XPA recruitment to the DNA damage sites in the nuclei of UV-irradated HeLa cells (Alekseev, S., et al. (2014). Chem Biol. 21(3):398-407).
Immunocytochemistry Analysis: A representative lot detected XPA recruitment to the DNA damage sites in the nuclei of UV-irradated HeLa cells (Alekseev, S., et al. (2014). Chem Biol. 21(3):398-407).
Quality
Evaluated by Western Blotting in HeLa nuclear extract.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected XPA in 10 µg of HeLa nuclear extract.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected XPA in 10 µg of HeLa nuclear extract.
Target description
~37 kDa observed. Target band appears larger than the calculated molecular weight of 31.4 kDa, most likely due to posttranslational modifications. Uncharacterized band(s) may appear in some lysates.
Physical form
Mouse monoclonal IgG1κ ascites with 0.05% sodium azide.
Unpurified
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Thomas H Sanderson et al.
PloS one, 8(11), e78627-e78627 (2013-11-14)
Recent advancements in isolation techniques for cytochrome c (Cytc) have allowed us to discover post-translational modifications of this protein. We previously identified two distinct tyrosine phosphorylated residues on Cytc in mammalian liver and heart that alter its electron transfer kinetics
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