Bcl2-associated athanogene 3 (BAG3), also known as CAIR-1 or Bis, belongs to a family of six evolutionarily conserved co-chaperone proteins that contain a characteristic C-terminal BAG domain. In most cell types, BAG3 is localized to the cytoplasm, where it regulates the heat shock protein 70 chaperone. BAG3 is activated in response to stressful stimuli, and plays a critical role in modulating multiple cellular processes, including apoptosis, autophagy, embryonic development, and actin rearrangement. Abnormal expression of BAG3 results cardiomyopathy dilated type 1HH.
Immunogen
MAP peptide corresponding to human BAG3.
Application
Anti-BAG3 Antibody, clone AC-2 detects level of BAG3 & has been published & validated for use in Western Blotting.
Immunoprecipitation Analysis: A 1:500 dilution from a representative lot detected BAG3 IP′d from 500 µg of HeLa lysate.
Research Category Apoptosis & Cancer
Research Sub Category Chaperones
Quality
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: A 1:1000 dilution of this antibody detected BAG3 in 10 µg of HeLa cell lysate.
Target description
~62-80 kDa observed. The calculated molecular weight of this protein is 62 kDa, but can run as high as 80 kDa due to PTM.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control HeLa cell lysate
Disclaimer
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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CRC is one of the most common malignancies worldwide, and its molecular mechanisms remain unclear. Elevated levels of BAG3 have been reported in various tumors. The present study aimed to explore the expression and function of BAG3 in CRC. BAG3
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