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Key Documents

MABC1189

Sigma-Aldrich

Anti-Bin3 Antibody, clone 3A4

clone 3A4, from mouse

Synonym(s):

Bridging integrator 3

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

3A4, monoclonal

species reactivity

mouse, human

packaging

antibody small pack of 25 μg

technique(s)

western blot: suitable

isotype

IgG1κ
IgG1

NCBI accession no.

UniProt accession no.

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... BIN3(55909)

Related Categories

General description

Bridging integrator 3 (UniProt: Q9NQY0; also known as Bin3) is encoded by the BIN3 gene (Gene ID: 55909) in human. Bin3 is a cytoskeletal protein that is ubiquitously expressed except in brain and is involved in cytokinesis and septation where it has a role in the localization of F-actin. It contain one BAR domain (aa 9-232), which forms coiled-coil structures. The BAR domain interacts with and facilitates tubulation of curved membranes and also binds to small GTPases and other cell regulatory proteins in the cytosol and nucleus. Deletion of Bin3 in mice is shown to cause cataracts associated with actin defects. Bin 3 I shown to suppress lymphoma during aging and restricts the efficiency of lung carcinogenesis. It is also shown to restrict the proliferation and motility of oncogenically transformed cells. (Ref.: Ramalingam, A., et al. (2008). Cancer Res. 68 (6): 1683-1690).

Specificity

Clone 3A4 detects Bridging integrator 3 (Bin3) in human and mouse cells.

Immunogen

GST-tagged full length recombinant human Bridging integrator 3 (Bin3).

Application

Anti-Bin3, clone 3A4, Cat. No. MABC1189, is a mouse monocloanl antibody that detects Bridging integrator 3 (Bin3) protein and has been tested for use in Western Blotting.
Detect using this mouse monoclonal Anti-Bin3, clone 3A4 Antibody, Cat. No. MABC1189-25UG, validated for use in Western Blotting.
Research Category
Apoptosis & Cancer
Western Blotting Analysis: 4 µg/mL from a representative lot detected Bin3 in 10 µg of MCF7 and HEK293 cell lysate.
Western Blotting Analysis: A representative lot detected Bin3 in Western Blotting applications (Ramalingam, A., et. al. (2008). Cancer Res. 68(6):1683-90).
Western Blotting Analysis: 4 µg/mL from a representative lot detected Bin3 in 10 µg of MCF7 and HEK293 cell lysate.

Western Blotting Analysis: A representative lot detected Bin3 in Western Blotting applications (Ramalingam, A., et. al. (2008). Cancer Res. 68(6):1683-90).

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 4 µg/mL of this antibody detected Bin3 in 10 µg of HeLa cell lysate.

Target description

~30 kDa observed; 29.66 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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